复合菌系预处理对玉米秸秆厌氧沼气发酵的影响  

EFFECT OF COMPLEX BACTERIA PRETREATMENT ON ANAEROBIC BIOGAS FERMENTATION OF CORN STRAW

作　　者：王丰园 金海炎 闫振丽 赵子高 鲁云风[1,2] Wang Fengyuan;Jin Haiyan;Yan Zhenli;Zhao Zigao;Lu Yunfeng(College of Life Science and Agricultural Engineering,Nanyang Normal University,Nanyang 473061,China;Henan University Biomass Resource Engineering Center,Nanyang 470361,China;State Key Laboratory of Motor Vehicle Biofuel Technology,Nanyang 450003,China)

机构地区：[1]南阳师范学院生命科学与农业工程学院,南阳473061 [2]河南省高校生物质资源化工程中心,南阳470361 [3]车用生物燃料技术国家重点实验室,南阳450003

出　　处：《太阳能学报》2023年第8期535-541,共7页Acta Energiae Solaris Sinica

基　　金：河南省高等学校重点科研项目(20A180022);车用生物燃料技术国家重点实验室开放课题(KFKT2018002)。

摘　　要：以玉米秸秆为原料,利用本实验室通过基因组重排育种后能高效降解木质纤维素的菌株对玉米秸秆进行预处理,并对预处理后的秸秆进行厌氧沼气发酵。通过正交试验确定复合菌系处理秸秆的最佳条件为:初始pH值为6.00、玉米秸秆含量为2.0%、TR2-35孢子液接种量1.0%、PR2-24孢子液接种量0.5%。通过对秸秆预处理发酵料液的定时检测,确定最佳的预处理时间为8 d,此时发酵液的pH值为6.82,适合厌氧产甲烷菌的生长,发酵液中的COD含量达到最大,为10080 mg/L,秸秆及其成分也达到降解的高峰,适合厌氧沼气发酵。通过设计1 L的厌氧沼气发酵排水集气装置,有效反应体积800 mL,进行20 d沼气发酵,结果表明:预处理组总产气量为6083 mL,总甲烷量为4316 mL,分别较未预处理组提高61.14%和93.63%。Using corn straw as raw material,corn straw was pretreated with the strains that can efficiently degrade lignocellulose after genome rearrangement breeding obtained in our laboratory,and the pretreated straw was fermented by anaerobic biogas.The optimum conditions for the treatment of straw by compound bacteria were determined by orthogonal test as follows:the initial pH value was 6.00,the content of corn straw was 2.0%,the inoculation amount of TR2-35 spore solution was 1.0%,and the inoculation amount of PR2-24 spore solution was 0.5%;the optimal pretreatment time was determined to be 8 d through the regular detection of the fermentation broth of straw pretreatment.At this time,the pH value of the fermentation broth was 6.82,which is suitable for the growth of anaerobic methanogens.The COD content in the fermentation broth reached the maximum value of 10080 mg/L.The straw and its components also reached the peak of degradation,which is suitable for anaerobic biogas fermentation.Through the design of 1 L anaerobic biogas fermentation drainage gas gathering device,the effective reaction volume was 800 mL,and the biogas fermentation was carried out for 20 d.The results show that the total gas production of the pretreatment group is 6083 mL,and the total methane production is 4316 mL,which are 61.14%and 93.63%higher than that of the non-pretreatment group respectively.

关 键 词：生物质能 沼气 厌氧发酵 复合菌系 预处理 玉米秸秆 

分 类 号：S216.4[农业科学—农业机械化工程]