Dissecting the genetic basis of grain color and pre-harvest sprouting resistance in common wheat by association analysis  

作　　者：YAN Sheng-nan YU Zhao-yu GAO Wei WANG Xu-yang CAO Jia-jia LU Jie MA Chuan-xi CHANG Cheng ZHANG Hai-ping 

机构地区：[1]Key Laboratory of Wheat Biology and Genetic Improvement on Southern Yellow&Huai River Valley,Ministry of Agriculture and Rural Affairs/College of Agronomy,Anhui Agricultural University,Hefei 230036,P.R.China

出　　处：《Journal of Integrative Agriculture》2023年第9期2617-2631,共15页农业科学学报（英文版）

基　　金：supported by grants from the University Synergy Innovation Program of Anhui Province,China(GXXT-2021-058);the National Natural Science Foundation of China(Joint Fund Projects,U20A2033);the Natural Science Foundation of Anhui Province,China(2108085MC98);the Jiangsu Collaborative Innovation Center for Modern Crop Production,China(JCIC-MCP);the key scientific and technological breakthroughs of Anhui Province(2021d06050003);the joint key project of improved wheat variety of Anhui Province,China(21803003).

摘　　要：Pre-harvest sprouting(PHS) adversely affects wheat quality and yield, and grain color(GC) is associated with PHS resistance. However, the genetic relationship between GC and PHS resistance remains unclear. In this study, 168 wheat varieties(lines) with significant differences in GC and PHS resistance were genotyped using an Illumina 90K iSelect SNP array. Genome-wide association study(GWAS) based on a mixed linear model showed that 67 marker-trait associations(MTAs) assigned to 29 loci, including 17 potentially novel loci, were significantly associated with GC, which explained 1.1–17.0% of the phenotypic variation. In addition, 100 MTAs belonging to 54 loci, including 31 novel loci, were significantly associated with PHS resistance, which accounted for 1.1–14.7% of the phenotypic variation. Subsequently, two cleaved amplified polymorphic sequences(CAPS) markers, 2B-448 on chromosome 2B and 5B-301 on chromosome 5B,were developed from the representative SNPs of the major common loci Qgc.ahau-2B.3/Qphs.ahau-2B.4controlling GC/PHS resistance and PHS resistance locus Qphs.ahau-5B.4, respectively. Further validation in 171 Chinese mini-core collections confirmed significant correlations of the two CAPS markers with GC and PHS resistance phenotypes under different environments(P<0.05). Furthermore, the wheat public expression database, transcriptomic sequencing, and gene allelic variation analysis identified TraesCS5B02G545100, which encodes glutaredoxin, as a potential candidate gene linked to Qphs.ahau-5B.4. The new CAPS marker CAPS-356 was then developed based on the SNP(T/C) in the coding sequences(CDS) region of TraesCS5B02G545100. The high-density linkage map of the Jing 411/Hongmangchun 21 recombinant inbred lines(RILs) constructed based on specific locus amplified fragment sequencing markers showed that CAPS-356 collocated with a novel QTL for PHS resistance, supporting the role of TraesCS5B02G545100 as the potential candidate gene linked to Qphs.ahau-5B.4. These results provide valuable information for the

关 键 词：common wheat grain color PHS resistance GWAS 90K SNP CAPS marker 

分 类 号：S512.1[农业科学—作物学]