电针预处理对脑缺血再灌注损伤大鼠神经功能及缺血半暗带区VEGF、CD31表达的影响  

Effects of electroacupuncture pretreatment on neurological function and expressions of VEGF and CD31 in the ischemic penumbra of rats with cerebral ischemia-reperfusion injury

作　　者：谭佳佳[1] 唐丽亚 孙晓莹 易细芹 瞿启睿 艾坤[1] 周璐[2] TAN Jiajia;TANG Liya;SUN Xiaoying;YI Xiqin;QU Qirui;AI Kun;ZHOU Lu(School of Acupuncture-moxibustion,Tuina and Rehabilitation,Hunan University of Chinese Medicine,Changsha,Hunan 410208,China;Department of Rehabilitation Medicine,Chenzhou First People's Hospital,Chenzhou,Hunan 423000,China)

机构地区：[1]湖南中医药大学针灸推拿与康复学院,湖南长沙410208 [2]郴州市第一人民医院康复医学科,湖南郴州423000

出　　处：《湖南中医药大学学报》2023年第9期1679-1684,共6页Journal of Hunan University of Chinese Medicine

基　　金：国家自然科学基金项目(82074565);湖南省自然科学基金青年项目(2021JJ40007);湖南中医药大学中西医结合一流学科开放基金项目(2020ZXYJH56)。

摘　　要：目的通过观察电针预处理对脑缺血再灌注损伤大鼠神经功能、脑梗死体积及半暗带区脑组织血管内皮生长因子(vascular endothelial growth factor,VEGF)、血小板-内皮细胞黏附分子(platelet endothelial cell adhesion molecule-1,CD31)表达的影响,判断电针预处理对半暗带区血管新生的影响,探索电针预处理改善脑缺血再灌注损伤的可能作用机制。方法将36只SD大鼠按照随机数字表法分为假手术组、模型组、电针预处理组,每组12只。电针预处理组予以电针百会、水沟、大椎,每天1次,共7次;假手术组、模型组仅捆绑,不予电针处理。实验第8天,参照Zea Longa方法对模型组、电针预处理组进行大脑动脉栓塞(medial cerebral artery occlusion,MCAO)造模后灌注,假手术组仅切开皮肤暴露颈动脉,不做手术。造模24 h后,对各组大鼠进行神经功能缺损评分(neurological severity scores,NSS)法评估神经功能,评分后取大鼠梗死侧脑半球行红四氮唑(2,3,5-Triphenyltetrazolium Chloride,TTC)染色检测脑梗死面积,取脑缺血半暗带区组织进行HE染色观察脑组织形态变化,免疫组织化学法测定VEGF、CD31表达。结果假手术组未见梗死以及任何神经功能损伤,细胞形态正常;模型组半暗带区细胞损伤显著增加,排列紊乱,呈肿胀状,毛细血管管腔塌陷;电针预处理组损伤程度减轻,排列轻度紊乱,细胞肿胀不明显。与假手术组比较,模型组大鼠神经功能缺损评分明显升高(P<0.01),脑梗死面积明显增加(P<0.01),缺血半暗带区VEGF、CD31表达明显增加(P<0.01);与模型组比较,电针预处理组大鼠神经功能缺损评分降低(P<0.05),脑梗死面积明显减少(P<0.01),缺血半暗带区VEGF、CD31表达明显增加(P<0.01)。结论电针预处理可能通过促进缺血半暗带区血管新生,从而改善脑缺血再灌注损伤大鼠神经功能损伤、降低脑梗死体积。Objective To evaluate the effects of electroacupuncture pretreatment on angiogenesis in the penumbra area and to explore the possible mechanism of action of electroacupuncture pretreatment in improving cerebral ischemia-reperfusion injury,by observing the effects of electroacupuncture pretreatment on the neurological function,cerebral infarction volume,and expressions of vascular endothelial growth factor(VEGF)and platelet endothelial cell adhesion molecule-1(CD31)in the ischemic penumbra orats with cerebral ischemia-reperfusion injury.Methods A total of 36 SD rats were randomly divided into sham-operated group,model group,and electroacupuncture pretreatment group by the random number table method,with 12 rats in each group.The electroacupuncture pretreatment group received electroacupuncture at"Baihui"(DU20),"Shuigou"(DU26),and"Dazhui"(DU13)once a day,with a total of 7 times;the sham-operated group and the model group were only bundled without electroacupuncture.On the 8th day of the experiment,according to the Zea Longa method,the model group and the electroacupuncture pretreatment group were subjected to medial cerebral artery occlusion(MCAO)modeling and reperfusion.The sham-operated group only underwent skin incision to expose the carotid artery and did not undergo surgery.After modeling for 24h,neurological severity scores(NSS)were applied to evaluate the neurological function of rats in each group.After scoring,the infarcted area was measured by 2,3,5-triphenyltetrazolium Chloride(TTC)staining in the affected cerebral hemisphere,the histomorphological changes of the tissue in the ischemic penumbra were observed by HE staining,and the expressions of VEGF and CD31 were determined by immunohistochemical method.Results There was no infarction or any neurological function injury in the sham-operated group,and the cellular morphology was normal;the cell damage in the penumbra area increased significantly in the model group,with a disordered arrangement and swollen appearance,and the capillary lumina collapsed;the

关 键 词：脑缺血再灌注 缺血半暗带 损伤 电针预处理 血管内皮生长因子 血小板-内皮细胞黏附分子 血管新生 

分 类 号：R245.9[医药卫生—针灸推拿学]