推拿■法对兔骨骼肌钝挫伤修复及Wnt/β-catenin信号通路的影响  

Effects of Tuina rolling manipulation on repair of skeletal muscle blunt contusion and Wnt/β-catenin signaling pathway in rabbit

作　　者：阮磊 黄博 王兰兰 薛惠天 孙梦龙 段苗苗 彭亮[1] RUAN Lei;HUANG Bo;WANG Lanlan;XUE Huitian;SUN Menglong;DUAN Miaomiao;PENG Liang(School of Acupuncture-moxibustion,Tuina and Rehabilitation,Hunan University of Chinese Medicine,Changsha,Hunan 410208,China)

机构地区：[1]湖南中医药大学针灸推拿与康复学院,湖南长沙410208

出　　处：《湖南中医药大学学报》2023年第9期1685-1692,共8页Journal of Hunan University of Chinese Medicine

基　　金：国家自然科学基金面上项目(82174521)。

摘　　要：目的探讨推拿■法对家兔骨骼肌钝挫伤修复及Wnt/β-catenin信号通路的影响。方法36只健康成年新西兰大耳白兔,随机分为对照组、模型组、■法组(每组12只),再根据不同取材时间点分为1、3、6 d 3个亚组(每组4只)。对照组实验全程正常饲养不予处理。模型组与■法组采用自制改良重力锤打击装置制备骨骼肌钝挫伤模型,于造模成功后7 d开始治疗,滚动频率140次/min,3 min/次,2次/d,共治疗3 d。分别于干预结束后1、3、6 d进行取材。通过HE和Masson染色观察家兔股四头肌病理改变;ELISA检测家兔血清中白细胞介素-6(interleukin 6,IL-6)和肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)表达;Western blot检测家兔股四头肌Wnt3α、β-catenin、糖原合成酶激酶-3β(glycogen synthase kinase-3β,GSK-3β)、磷酸化糖原合成酶激酶-3β(phosphorylated glycogen synthase kinase-3β,p-GSK-3β)、转化生长因子-β1(transforming growth factor-β1,TGF-β1)、Ⅰ型胶原蛋白(CollagenⅠ,COL-Ⅰ)表达。结果(1)HE和Masson染色结果显示:对照组可见肌纤维排列规整有序,结构完整;模型组肌纤维大小、粗细、形态不一,排列紊乱,间隙增宽,炎细胞浸润、结缔组织增生以及胶原纤维沉积明显;■法组肌纤维无明显变性坏死,结构相对完整,可见少量炎细胞浸润,结缔组织增生与胶原纤维沉积皆明显减少,修复优于模型组。(2)ELISA与Western blot检测结果:与对照组相比,模型组家兔血清IL-6、TNF-α以及股四头肌中Wnt3a、β-catenin、p-GSK-3β、TGF-β1、COL-Ⅰ表达量在1、3、6 d均显著升高(P<0.01),GSK-3β表达量显著降低(P<0.01);与模型组比较,■法组于1、3、6 d家兔血清IL-6、TNF-α与股四头肌中Wnt3a、β-catenin、p-GSK-3β、TGF-β1、COL-Ⅰ表达量皆明显降低(P<0.01),GSK-3β表达量明显升高(P<0.05,P<0.01)。结论推拿■法能有效减轻骨骼肌损伤后体内炎症反应,减少胶原蛋白沉积,抑制骨骼肌纤维�Objective To explore the effects of Tuina rolling manipulation on the repair of skeletal muscle blunt contusion and Wnt/β-catenin signaling pathway in rabbit.Methods A total of 36 healthy adult New Zealand big-eared white rabbits were randomly divided into a control group,a model group,and a rolling group(12 rabbits in each group),and then divided into 3 subgroups(4 rabbits in each group)according to the different time points of sampling,namely,1 d,3 d,and 6 d..The control group was fed normally throughout the entire experimental period without treatment.The skeletal muscle blunt contusion model was prepared by self-made improved gravity hammer percussion device in model group and rolling manipulation group,and the treatment started 7 days after the successful modeling,with a frequency and duration of 140 rolls/min,3 min/time,and 2 times/d,for a total of 3 days.Samples were taken on 1d,3d,and 6d after the intervention.HE and Masson staining were used to observe the pathological changes of quadriceps femoris in rabbits;the expressions of interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)in rabbit serum were checked by ELISA;Western-Blot was used to measure the protein expressions of Wnt3a,β-catenin,glycogen synthase kinase-3β(GSK-3β),phosphorylated glycogen synthase kinase-3β(p-GSK-3β),transforming growth factor-β1(TGF-β1)and CollagenⅠ(COL-I)in the muscles.Results(1)The results of HE and Masson staining showed that:the muscle fibers of the quadriceps femoris of rabbits in the control group were well arranged and structurally intact;those in the model group were of different sizes,thicknesses and forms,and were arranged in a disordered manner,with widened gaps as well as obvious inflammatory cell infiltration,connective tissue proliferation,and collagen fiber deposition;those in the rolling manipulation group showed no obvious degeneration and necrosis,and the structures were relatively intact with a low degree of inflammatory cell infiltration,and the connective tissue proliferation and collagen fib

关 键 词：推拿■法 骨骼肌钝挫伤 炎症 骨骼肌纤维化 骨骼肌损伤修复 WNT/Β-CATENIN信号通路 机制 

分 类 号：R244.1[医药卫生—针灸推拿学]