短冠东风菜的化学成分研究  

Study on the Chemical Constituents of Duanguan Dongfengcai(Doellingeria Marchandii)

作　　者：谢洁兰 莫小莹 陈晓春 李耀华[1] 黄秋燕 梁平 罗威 潘小姣[1,4] XIE Jielan;MO Xiaoying;CHEN Xiaochun;LI Yaohua;HUANG Qiuyan;LIANG Ping;LUO Wei;PAN Xiaojiao(Guangxi University of Chinese Medicine,Nanning Guangxi 530200,China;Wuzhou Hospital of Traditional Chinese Medicine,Wuzhou Guangxi 543003,China;International Biotoxin Research Center,Wuzhou Guangxi 543003,China;Key Laboratory of Extraction,Purification and Quality Analysis of Traditional Chinese Medicine in Guangxi University,Nanning Guangxi 530200,China)

机构地区：[1]广西中医药大学,广西南宁530200 [2]梧州市中医医院,广西梧州543003 [3]国际生物毒素研究中心,广西梧州543003 [4]广西高校中药提取纯化与质量分析重点实验室,广西南宁530200

出　　处：《中医药导报》2023年第9期38-42,共5页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：广西中医药管理局、药品监督管理局资助项目(GZZJ202029,GZZJ202030)。

摘　　要：目的:研究短冠东风菜的化学成分。方法:采用水蒸气蒸馏法提取挥发油,并用气相色谱-质谱联用法(GC-MS)分析其化学成分;通过硅胶柱色谱和制备高效液相色谱法对乙醇提取物进行分离纯化,根据波谱数据与文献对照鉴定化合物的化学结构。结果:从挥发油中鉴定出14个化学成分,其主要化学成分为石竹烯氧化物(26.68%);从乙醇提取物中分离得到15个化合物,分别鉴定为:3-O-β-D-葡萄糖醛酸吡喃基-齐墩果酸甲酯(1)、杨梅素(2)、槲皮素(3)、山奈酚(4)、芦丁(5)、紫云英苷(6)、异槲皮苷(7)、异绿原酸A(8)、异绿原酸B(9)、异绿原酸C(10)、绿原酸(11)、隐绿原酸(12)、新绿原酸(13)、咖啡酸(14)、没食子酸(15)。结论:挥发油中含量最高的是石竹烯氧化物;所有化合物均为首次从该植物中分离得到。Objective:To study the chemical constituents of Duanguan Dongfengcai(Doellingeria Marchandii).Methods:The volatile oil was extracted by steam distillation and analyzed by GC-MS.The ethanol extracts were separated and purified by silica gel column chromatography and preparative high performance liquid chromatography.The structures of the compounds were identified according to the spectral data and literature.Results:A total of 14 volatile components were identified by GC-MS and the main volatile chemical composition was caryophyllene oxide(26.68%).A total of 15 compounds were isolated from the ethanol extracts and identified as 3-O-β-D-glucuronopyranosyl oleanolic acid methyl ester(1),myricetin(2),quercetin(3),kaempferol(4),rutin(5),astragaloside(6),isoquercetin(7),isochlorogenic acid A(8),isochlorogenic acid B(9),isochlorogenic acid C(10),chlorogenic acid(11)cryptochlorogenic acid(12),neochlorogenic acid(13),caffeic acid(14),gallic acid(15).Conclusion:The highest content of volatile oil is caryophyllene oxide.All compounds were isolated from this plant for the first time.

关 键 词：短冠东风菜 化学成分 石竹烯氧化物 咖啡酰基奎宁酸 气相色谱-质谱联用法 

分 类 号：R284.1[医药卫生—中药学]