阿片类药物对围术期微生物组及认知功能变化的影响  

Effects of Opioids on Perioperative Microbiome and Cognitive Function Changes

作　　者：李冰斯 牛骊 刘丽 Li Bingsi;Niu Li;Liu Li(Heilongjiang Red Cross(Sengong General)Hospital,Harbin,Heilongjiang,150000,China)

机构地区：[1]黑龙江省红十字(森工总)医院,黑龙江哈尔滨150000 [2]迎春林业地区人民医院,黑龙江虎林154300

出　　处：《黑龙江医学》2023年第19期2309-2312,共4页Heilongjiang Medical Journal

基　　金：黑龙江省卫健委科研课题(20210404110311)。

摘　　要：目的:通过对使用吗啡小鼠的细菌16s rDNA测序,探讨使用吗啡等阿片药物对围术期微生物组和认知功能变化的影响。方法:采用随机数表法将40只小鼠分为4组,每组各10只,包括吗啡模型组(M组),安慰剂对照组(C组),吗啡模型+安慰剂对照粪菌移植组(MC组),安慰剂对照+吗啡模型粪菌移植组(CM组)。通过动物造模,粪菌液制备,动物取样之后进行16s RNA测序。以镇痛剂量向C57BL6小鼠施用约1μM血清浓度的吗啡,72 h后收集肠道粪便内容物并分析微生物组成。结果:在对照组和吗啡干预的动物之间可观察到肠道微生物组成的显著变化,吗啡暴露可能会增加黏膜感染的易感性,可降低病原体清除率,并诱导细菌穿过肠道屏障移位。吗啡显著改变了肠道微生物组成,并诱导革兰氏阳性病原菌的优先扩增。吗啡引起的微生态失调可以通过移植对照组小鼠的微生物群来逆转。结论:围术期微生物组调节可能成为控制吗啡副作用的治疗策略,并影响围术期认知障碍。Objective:To investigate the effects of the use of opioid drugs such as morphine on perioperative changes in the mi⁃crobiome and cognitive function by sequencing bacterial 16s rDNA from mice on morphine.Methods:The random number table method was used to divide 40 mice into 4 groups,with 10 mice in each group,including morphine model group(Group M),placebo control group(Group C),morphine model+placebo control fecal colony transplantation group(Group MC),and placebo control+morphine model fecal colony transplantation group(Group CM).16s RNA sequencing was performed by animal modeling,fecal fluid preparation,and animal sampling followed by 16s RNA sequencing.Morphine at a serum concentration of approximately 1μM was administered to C57BL6 mice at an analgesic dose,and intestinal fecal contents were collected and analyzed for microbial composi⁃tion 72 h later.Results:Significant changes in gut microbial composition were observed between control and morphine-intervened animals,and morphine exposure may increase susceptibility to mucosal infections,may decrease pathogen clearance,and induce bacterial translocation across the intestinal barrier.Morphine significantly altered gut microbial composition and induced preferential amplification of Gram-positive pathogenic bacteria.Morphine-induced microecological dysregulation could be re⁃versed by transplanting the microbiota of control mice.Conclusion:Perioperative microbiome modulation may be a therapeutic strategy to control morphine side effects and influence perioperative cognitive impairment.

关 键 词：吗啡 肠道菌群 粪菌移植 

分 类 号：R614[医药卫生—麻醉学]