棉花查尔酮异构酶基因家族的鉴定及表达分析  

Identification and expression analysis of chalcone isomerase gene family in cotton

作　　者：杨丹 鲍军秀 陈沛 王斐[1,2] 汤寿伍[3] 刘海峰 李鸿彬 YANG Dan;BAO Junxiu;CHEN Pei;WANG Fei;TANG Shouwu;LIU Haifeng;LI Hongbin(College of Life Sciences,Shihezi University,Shihezi 832003,China;Key Laboratory of Oasis Town and Mountain Basin System Ecology,Xinjiang Production and Construction Group/Key Laboratory of Xinjiang Phytomedicine Resource and Utilization,Ministry of Education,Shihezi 832000,China;China Colored-Cotton(Group)Co.,Ltd.,Urumqi 830001,China)

机构地区：[1]石河子大学生命科学学院,石河子832003 [2]绿洲城镇与山盆系统生态兵团重点实验室/新疆植物药资源利用教育部重点实验室,石河子832000 [3]中国彩棉(集团)股份有限公司,乌鲁木齐830001

出　　处：《华中农业大学学报》2023年第5期94-104,共11页Journal of Huazhong Agricultural University

基　　金：新疆维吾尔自治区创新环境建设专项(2020D14035);新疆生产建设兵团科技攻关与成果转化项目(2016AC017)。

摘　　要：为研究陆地棉查尔酮异构酶CHI(chalcone isomerase)在彩色棉纤维发育及响应胁迫中的作用,对陆地棉基因组中GhCHI基因家族进行鉴定,开展蛋白理化性质、结构域、启动子顺式作用元件、蛋白质高级结构、系统进化分析,利用转录组数据和实时荧光定量PCR分析其表达特征。结果显示,从陆地棉基因组中鉴定获得12个GhCHI基因家族成员,可分为2个亚家族,具有典型的查尔酮超家族结构域,编码201~452个氨基酸,主要为亲水性蛋白,二级结构主要以α-螺旋和无规则卷曲为主。GhCHI基因启动子顺式作用元件类型主要包含光反应元件、激素响应元件及参与类黄酮生物合成调控的元件等。表达分析结果显示GhCHI1、GhCHI2、GhCHI3、GhCHI4与纤维发育密切相关,尤其在彩色棉纤维发育后期的色素沉积着色时期表达水平较高;这4个基因在叶、花托、雌蕊中也具有较高的表达水平;GhCHI1、GhCHI2、GhCHI3在热胁迫、盐胁迫和干旱胁迫下均受到显著的诱导表达。GhCHI1―GhCHI4蛋白互作分析结果显示,它们可能和参与类黄酮合成的2-氧代戊二酸3-双加氧酶和类黄酮3´-单加氧酶等蛋白质存在相互作用。结果表明,GhCHI1、GhCHI2、GhCHI3和GhCHI4基因在彩色棉的纤维发育和胁迫应答中发挥重要作用。To study the function of chalcone isomerase(CHI)gene in fiber development and re⁃sponse to stress in colored cotton,the physicochemical properties,conserved domain,cis-acting elements in the promoter of CHI gene,advanced structures and phylogeny of proteins were analyzed,transcriptome data and quantitative real-time PCR(qRT-PCR)were used to analyze its expression profile.The results showed that 12 members of GhCHI gene family were identified from the genome of upland cotton,which can be divided into two subfamilies with typical chalcone superfamily domains,encoding 201-452 amino ac⁃ids,mainly hydrophilic proteins,and secondary structures mainly composed ofα-helix and irregular curls.The types of cis-acting elements in the promoter of GhCHI gene mainly include light response elements,hormone response elements,and elements involved in the regulation of flavonoid biosynthesis.The results of analyzing expression profile showed that GhCHI1,GhCHI2,GhCHI3,and GhCHI4 were closely related to fiber development,especially with high level of expression during the period of pigmentation and coloring at the later stage of fiber development in colored cotton.These four genes also had high level of expression in leaves,receptacles,and pistils.GhCHI1,GhCHI2,and GhCHI3 were significantly induced to express under heat stress,salt stress,and drought stress.The results of qRT-PCR showed that GhCHI1-GhCHI4 were significantly enriched in fiber growth,especially in colored cotton.The results of analyzing the interaction of GhCHI1-GhCHI4 protein showed that they may interact with proteins such as 2-oxoglutarate 3-dioxygen⁃ase and flavonoid 3´-monooxygenase involved in the flavonoid synthesis.It is indicated that GhCHI1,Gh⁃CHI2,GhCHI3,and GhCHI4 genes play important roles in fiber development and stress response in col⁃ored cotton.

关 键 词：天然彩色棉 查尔酮异构酶 基因家族 纤维发育 色素沉积 

分 类 号：S562[农业科学—作物学]