High‐throughput formation of miniaturized cocultures of 2D cell monolayers and 3D cell spheroids using droplet microarray  

作　　者：Haijun Cui Tina Tronser Xianxian Wang Janine Wesslowski Gary Davidson Anna A.Popova Pavel A.Levkin 

机构地区：[1]Institute of Biological and Chemical Systems‐Functional Molecular Systems(IBCS‐FMS),Karlsruhe Institute of Technology(KIT),Eggenstein‐Leopoldshafen,Germany [2]Key Laboratory of Biorheological Science and Technology,Ministry of Education,College of Bioengineering,Chongqing University,Chongqing,China [3]Institute of Organic Chemistry,Karlsruhe Institute of Technology(KIT),Karlsruhe,Germany

出　　处：《Droplet》2023年第1期36-46,共11页液滴（英文）

基　　金：Deutsche Forschungsgemeinschaft(DFG,German Research Foundation),Grant/Award Numbers:406232485,LE 2936/9‐1,331351713–SFB1324;Heidelberg Karlsruhe Strategic Partnership(HeiKa,Germany);Impuls‐und Vernetzungsfonds der Helmholtz‐Gemeinschaft。

摘　　要：Most of the biological processes,including cell signaling,cancer invasion,embryogenesis,or neural development,are dependent on and guided by the complex architecture and composition of cellular microenvironments.Mimicking such microenvironments in cell coculture models is crucial for fundamental and applied biology investigations.The ability to combine different cell types grown as both two‐dimensional(2D)monolayers and three‐dimensional(3D)spheroids in specific defined location inside a microculture environments is a key towards in vitro tissue modeling and towards mimicking complex in vivo cellular processes.In this study,we introduce and investigate a method to create in vitro models of 2D cell monolayers cocultured with 3D spheroids in defined preorganization.We demonstrate the possibility of creating such complex cellular microenvironments in a high‐throughput and automated manner by creating arrays of such droplets containing prearranged 2D and 3D cellular microcolonies.Furthermore,we demonstrate an application of this approach to study paracrine propagation of Wnt signaling between 2D and 3D cellular colonies.This method provides a general approach for the miniaturized,high‐throughput,and automated formation of complex coculture cellular microarchitectures that will be useful for mimicking various in vivo complex cellular structures and for studying complex biological processes in vitro.

关 键 词：INVASION architectures COCULTURE 

分 类 号：R318[医药卫生—生物医学工程]