A fluorescent film sensor for high-performance detection of Listeria monocytogenes via vapor sampling  

作　　者：Rongrong Huang Meiqi Li Dehui Lin Yangtao Shao Congdi Shang Qianqian Liu Guijun Liu Nan Li Rong Miao Haonan Peng Yanli Tang Yu Fang 

机构地区：[1]Key Laboratory of Applied Surface and Colloid Chemistry(Ministry of Education),School of Chemistry and Chemical Engineering,Shaanxi Normal University,Xi’an,China [2]Shaanxi Engineering Laboratory for Food Green Processing and Safety Control,College of Food Engineering and Nutritional Science,Shaanxi Normal University,Xi’an,China [3]Department of Food Safety,College of Food Science and Engineering,Northwest A&F University,Shaanxi,China

出　　处：《Aggregate》2023年第1期165-172,共8页聚集体（英文）

基　　金：National Natural Science Foundation of China,Grant/Award Numbers:21820102005,22132002;111 Project,Grant/Award Number:B14041;Fundamental Research Funds for the Central Universities,Grant/Award Number:2019CBLY001。

摘　　要：Foodborne Listeria monocytogenes poses serious threats to public health.Fast and sensitive detection of the pathogen at the point of contamination is thus crucial to halt the spread of bacteria-related diseases.Herein,we report for the first time a fluorescent film sensor to detect the biomarker of L.monocytogenes,3-hydroxy-2-butanone.The sensor demonstrated unprecedented sensing performance for the analyte with a detection limit lower than 0.05 mg/m^(3),response time less than 1 s,full reversibility,and excellent selectivity.Further study showed that the sensor can be used to monitor the growth of L.monocytogenes with much-improved sensitivity.The superior performance of the sensor is ascribed to the specific binding,efficient charge transfer emission,and porous adlayer structure of the specially designed sensing fluorophore-based film.Our work paves the way to develop a portable detector to meet the needs for on-site and real-time detection of foodborne pathogens.

关 键 词：3-hydroxy-2-butanone biomarkers fluorescent film sensors Listeria monocytogenes 

分 类 号：TP212[自动化与计算机技术—检测技术与自动化装置]