Site-specific fabrication of gold nanocluster-based fluorescence photoswitch enabled by the dual roles of albumin proteins  

作　　者：Wencheng Zhong Xiaojian Yan Shaohua Qu Li Shang 

机构地区：[1]State Key Laboratory of Solidification Processing,School of Materials Science and Engineering,Northwestern Polytechnical University and Shaanxi Joint Laboratory of Graphene(NPU),Xi’an,China [2]Northwestern Polytechnical University Chongqing Technology Innovation Center,Chongqing,China [3]NPU-QMUL Joint Research Institute of Advanced Materials and Structures(JRI-AMAS),Northwestern Polytechnical University,Xi’an,China

出　　处：《Aggregate》2023年第1期268-276,共9页聚集体（英文）

基　　金：Natural Science Foundation of Chongqing,Grant/Award Number:cstc2021jcyj-msxmX0980;Fundamental Research Fund for the Central University,Grant/Award Number:3102019jcc005;Innovation Foundation for Doctor Dissertation of Northwestern Polytechnical University,Grant/Award Number:CX2021053;Research Fund of the State Key Laboratory of Solidification Processing,Grant/Award Number:2020-QZ-01。

摘　　要：Fluorescence photoswitch is becoming increasingly desirable for many applications,but its controllable fabrication still remains challenging yet.In this paper,a new strategy is proposed to fabricate fluorescence photoswitch by harnessing dual roles of albumin proteins as both photochrome carriers and biotemplates of fluorophore.As an example,we demonstrated the successful fabrication of a fluorescence photoswitch by incorporating both the photochromic diarylethene dye(CMTE)and red-emitting fluorescent gold nanoclusters(AuNCs)into the specific domains of bovine serum albumin(BSA)in a highly controllable manner.Detailed spectral and photophysical characterisation showed that CMTE well-retains the photochromic properties within the CMTE–BSA–AuNC construct,although its photoconversion rate is slightly retarded.Different from previously reported photoswitches,the fluorescence of the present system is mainly modulated via the inner filter effect(IFE)mechanism,which exhibits high switching efficiency with an on-off ratio up to 90%,reversible fluorescence response and good antifatigue performance.This work provides a new,generable albumin protein-assisted strategy of fabricating advanced fluorescence photoswitch,which can find wide applications in biological,optical and information fields.

关 键 词：albumin protein fluorescence switch gold nanoclusters inner filter effect photochrome 

分 类 号：O657.3[理学—分析化学]