A NIR ratiometric fluorescent biosensor for sensitive detection and imaging of α-L-fucosidase in living cells and HCC tumor-bearing mice  

作　　者：Guo-Qiang Zhang Weiwei Feng Zhiyuan Gao Gao-Lan Zhang Xinying Wu Yating Xiao Xun Li Lei Zheng Dan Ding Jianshuang Guo Bo Situ 

机构地区：[1]Frontiers Science Center for Cell Responses,State Key Laboratory of Medicinal Chemical Biology Key Laboratory of Bioactive Materials,Ministry of Education,and College of Life Sciences,Nankai University,Tianjin,China [2]Department of Laboratory Medicine,Nanfang Hospital,Southern Medical University,Guangzhou,China [3]Department of Pharmacology and Molecular Sciences,Johns Hopkins University School of Medicine,Baltimore,Maryland,USA [4]School of Molecular Medicine,Hangzhou Institute for Advanced Study,UCAS,Hangzhou,China [5]School of Chemical Engineering,University of Chinese academy of science,Beijing,China

出　　处：《Aggregate》2023年第2期300-308,共9页聚集体（英文）

基　　金：National Key R&D Program of China,Grant/Award Number:2017YFE0132200;National Natural Science Foundation of China,Grant/Award Numbers:5191160730,51873092,81921004,81802116;Natural Science Foundation of Guangdong Province,Grant/Award Number:2021A1515011633;Science and Technology Program of Guangzhou,Grant/Award Number:202102020705;Outstanding Youths Development Scheme of Nanfang Hospital,Southern Medical University,Grant/Award Number:2018J002;Tianjin Science Fund for Distinguished Young Scholars,Grant/Award Number:19JCQJC61200。

摘　　要：Detection and imaging of α-L-fucosidase(AFU)is of great value to understand its roles in hepatocellular carcinoma(HCC)and tumor early diagnosis,but ideal assays are still lacking.Herein,a near-infrared(NIR)fluorescent biosensor(α-Fuc-DCM)was elaborately designed and synthesized for rapid and ratiometric detection of AFU activity in cells and HCC tumor mouse models.In the presence of AFU,this biosensor shows an enhancement in NIR emission in a ratiometric manner,which significantly improves the detection accuracy with the limit of detection as low as 4.8 mU/mL.Taking advantage of these merits,the activity of AFU in lysosomes could be visualized using ratiometric and NIR dual modality in living cells.Furthermore,its remarkable application for monitoring of endogenous AFU activity in HCC tumor-bearing mouse model is also demonstrated with bright fluorescence signal,which indicated that the biosensor could clearly monitor the liver tumor in the early stage.Importantly,the α-Fuc-DCM probe can be utilized to detect the AFU in serum from HCC patients.This strategy offers a promising biosensor system for early diagnosis of HCC and studying the roles of AFU in cancers.

关 键 词：cell and tumor imaging NIR fluorescent nanoprobe Α-L-FUCOSIDASE 

分 类 号：O657.3[理学—分析化学]