An activatable NIR-Ⅱ fluorescent probe for tracking heavy-metal ion and high-level salt-induced oxidative stress in plant sprouts  

作　　者：Yi Long Junjie Chen Fang Zeng Shuizhu Wu 

机构地区：[1]Biomedical Division,State Key Laboratory of Luminescent Materials and Devices,Guangdong Provincial Key Laboratory of Luminescence from Molecular Aggregates,College of Materials Science and Engineering,South China University of Technology,Guangzhou,China

出　　处：《Aggregate》2023年第2期309-317,共9页聚集体（英文）

基　　金：NSFC,Grant/Award Numbers:21788102,21875069;the Fund of Guangdong Provincial Key Laboratory of Luminescence from Molecular Aggregates,Grant/Award Number:2019B030301003。

摘　　要：Humans and plants have become enfolded and inseparable.Abiotic stresses in particular oxidative stress caused by heavy-metal ions or high-level salt contamination deleteriously impact plants’growth process and have become a major threat to sustaining food security.Sprouting is the first step in plants’growth process.When plant sprouts endure oxidative stress induced by toxic heavy-metal ions or high-level salt,accelerated generation of reactive oxygen species(e.g.,H_(2)O_(2))occurs inside plant sprouts;hence in-situ H_(2)O_(2) in plant sprouts could serve as the in-vivo biomarker for tracking the oxidative stress in plant sprouts.Herein,we design an activatable probe CT-XA-H_(2)O_(2) to track the oxidative stress in plant sprouts via in vivo NIR-Ⅱ fluorescent imaging.In CT-XA-H_(2)O_(2),cyano-thiazole acts as the electron-accepting moiety and xanthane-aminodiphenyl as the electron-donating moiety,and dioxaborolane as the biomarker-responsive unit and fluorescence quencher.The probe CT-XA-H_(2)O_(2) shows weak fluorescent emission.When H_(2)O_(2) is present,the dioxaborolane in the probe is cleaved,consequently,the dye CT-XA-OH is generated and brings about significant fluorescent signals for detecting and imaging the in-situ biomarker.Moreover,the aminodiphenyl group endues the chromophore(the activated probe)with aggregation-induced emission characteristics,which ensures stronger fluorescence in the aggregated state in the aqueous milieu.The probe CT-XA-H_(2)O_(2) has been employed in the Cd^(2+)-ion or high-level salt(NaCl)induced oxidative stress models of soybean sprouts and peanut sprouts,and the experimental results evidently reveal the probe’s ability for in-situ biomarker-activatable in-vivo detection and imaging in the plants’sprouts.

关 键 词：activatable probe aggregation-induced emission fluorescent probe near-infrared second window fluorescence imaging oxidative stress SPROUT 

分 类 号：O657.3[理学—分析化学]