糖尿病肾病继发医院感染患者外周血IL-6、STAT3、VEGF表达及意义  

Expressions of IL-6,STAT3 and VEGF in peripheral blood of diabetic nephropathy patients complicated with nosocomial infection and their significance

作　　者：卢懿[1] 魏冉 彭丽 李素荣 王晶[4] 郑领涛 石振峰[1] 张军英[1] LU Yi;WEI Ran;PENG Li;LI Su-rong;WANG Jing;ZHENG Ling-tao;SHI Zhen-feng;ZHANG Jun-ying(Xingtai People's Hospital,Xingtai,Hebei 05400l,China)

机构地区：[1]邢台市人民医院内分泌科,河北邢台054001 [2]邢台市人民医院内分泌实验室,河北邢台054001 [3]邢台市人民医院疼痛科,河北邢台054001 [4]邢台市人民医院肾脏内科,河北邢台054001

出　　处：《中华医院感染学杂志》2023年第17期2610-2614,共5页Chinese Journal of Nosocomiology

基　　金：邢台市科技计划项目(2018ZC208)。

摘　　要：目的探讨糖尿病肾病继发医院感染患者外周血白细胞介素-6(IL-6)、信号传导与转录激活因子3(STAT3)、血管内皮生长因子(VEGF)表达及意义.方法选取2018年11月-2019年12月邢台市人民医院收治的糖尿病肾病继发医院感染患者26例为感染组,按1∶5比例选择同期未发生医院感染的糖尿病肾病患者130例为未感染组,收集感染组患者临床标本进行病原菌鉴定,抽取患者外周血,检测血清IL-6、VEGF水平及外周血单个核细胞(PBMC)中STAT3 mRNA相对表达量,受试者工作特征(ROC)曲线评估诊断感染的效能.结果26例糖尿病肾病继发医院感染患者送检标本共分离出33株病原菌,主要为铜绿假单胞菌(24.24%)、金黄色葡萄球菌(21.21%)、大肠埃希菌(15.16%)和肺炎克雷伯菌(12.12%);感染组患者外周血IL-6、VEGF水平和PBMC中STAT3 mRNA相对表达量均高于未感染组(P<0.05);ROC曲线分析结果显示,IL-6、STAT3和VEGF表达诊断糖尿病肾病继发医院感染的曲线下面积(AUC)分别为0.860、0.775和0.728,均>0.7,预测效能较高.结论糖尿病肾病继发医院感染患者外周血中IL-6、STAT3和VEGF的表达水平均升高,且诊断医院感染的效能均较高.OBJECTIVE To explore the expressions of interleukin-6(IL-6),signal transducer and activator of transcription 3(STAT3)and vascular endothelial growth factor(VEGF)in peripheral blood of diabetic nephropathy patients complicated with nosocomial infection and analyze the significance.METHODS A total of 26 diabetic nephropathy patients who were complicated with nosocomial infection and treated in Xingtai People's Hospital from Nov 2018 to Dec 2019 were assigned as the infection group,meanwhile,130 diabetic nephropathy patients who were not complicated with nosocomial infection were chosen in a 1:5 ratio and assigned as the no infection group.The clinical specimens were collected from the patients of the infection group,the isolated pathogens were identified,the peripheral blood specimens were extracted from the patients,the levels of serum IL-6,VEGF and the relative expression level of STAT3 mRNA in peripheral blood menonuclear cell(PBMC)was detected,and the efficiencies of the indexes in diagnosis of infection were evaluated by receiver operating characteristic(ROC)curve.RESULTS Totally 33 strains of pathogens were isolated from the submitted specimens of the 26 diabetic nephropathy patients complicated with nosocomial infection;Pseudomonas aeruginosa(24.24%),Staphylococcus aureus(21.21%)and Klebsiella pneumoniae(12.12%)were dominant among the isolated pathogens.The levels of peripheral blood IL-6,VEGF and the relative expression level of STAT3 mRNA in PBMC were higher in the infection group than in the no infection group P<0.05).ROC curve analysis showed that the areas under curves(AUCs)of IL-6,STAT3 and VEGF were respectively 0.860,0.775 and 0.728 in diagnosis of the complicated nosocomial infection in the diabetic nephropathy patients,all of them were greater than O.7,with the predictive efficiencies high.CONCLUSION The expression levels of peripheral blood IL-6,STAT3 and VEGF of the diabetic nephropathy patients complicated with nosocomial infection are elevated,and the efficiencies are high in diagnosis of nosoc

关 键 词：糖尿病肾病 医院感染 白细胞介素-6 信号传导与转录激活因子3 血管内皮生长因子 

分 类 号：R587.2[医药卫生—内分泌]