核黄素参与水稻非生物胁迫的响应分析  

Analysis of Riboflavin Involvement in Abiotic Stress Response in Rice

作　　者：夏清珊 周正琨 谭炎宁[2] 刘选明[1] 秦迪岚 林建中[1] XIA Qingshan;ZHOU Zhengkun;TAN Yanning;LIU Xuanming;QIN Dilan;LIN Jianzhong(Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation/National Center of Technology Innovation for Saline-Alkali Tolerant Rice,College of Biology,Hunan University,Changsha 410082,China;Hunan Hybrid Rice Research Center,Changsha 410125,China;State Environmental Protection Key Laboratory of Monitoring for Heavy Metal Pollutions,Hunan Ecological and Environmental Monitoring Center,Changsha 410019,China)

机构地区：[1]湖南大学生物学院植物功能基因组学和发育调控湖南省重点实验室/国家耐盐碱水稻技术创新中心,长沙410082 [2]湖南杂交水稻研究中心,长沙410125 [3]湖南省生态环境监测中心国家环境保护重金属污染监测重点实验室,长沙410019

出　　处：《西北植物学报》2023年第8期1314-1322,共9页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金资助项目(32272139,31871595);湖南省自然科学基金资助项目(2020JJ4004);杂交水稻国家重点实验室(湖南杂交水稻研究中心)开放课题项目(2019KF02);海南省崖州湾种子实验室揭榜挂帅项目(B21HJ0108)。

摘　　要：为探究核黄素在水稻非生物胁迫响应中的作用,以粳稻Kitaake和籼稻T98B为试验材料,考察了核黄素对2种材料的盐、高温、渗透、碱和氧化胁迫响应的影响,重点测定了盐和高温胁迫下水稻体内核黄素合成基因的表达和相关生理指标。结果表明,(1)施加外源核黄素有效提高了2种水稻材料的盐和高温胁迫耐受性,降低了渗透胁迫耐受性,而其氧化和碱胁迫耐受性不受影响。(2)逆境胁迫均不同程度地促进了核黄素在2种水稻材料中的积累,尤其在盐和高温胁迫下促进效果最明显。(3)盐和高温胁迫均诱导了核黄素合成酶基因的表达,促进了核黄素的生物合成,改善了水稻的胁迫耐受性。研究表明,非生物逆境胁迫能促进核黄素在水稻体内的合成和积累,外源核黄素也能明显提高水稻对盐和高温胁迫的耐受性,但却降低了其对渗透胁迫的耐受性。In order to explore the role of riboflavin in response to abiotic stress in rice(Oryza sativa L.),a japonica rice Kitaake and an indica rice T98B were used as experimental materials to investigate the effects of riboflavin on their response to salt,high temperature,osmotic,alkali,and oxidative stresses.The expression levels of riboflavin biosynthesis genes and stress related physiological indexes were determined intensively in rice under salt and high temperature stresses.The results demonstrated that:(1)Exogenous riboflavin effectively enhanced the tolerance to salt and high temperature stresses,and reduced the tolerance to osmotic stress,but did not affect the tolerance to oxidative and alkali stresses.(2)All the abiotic stresses,especially salt and high temperature,promoted the accumulation of riboflavin to different degrees in rice.(3)Both salt and high temperature stresses induced the expression of riboflavin biosynthesis genes,which promoted the biosynthesis of riboflavin and improved the stress tolerance in rice.These results indicate that abiotic stress could promote the biosynthesis and accumulation of riboflavin in rice,and riboflavin could also enhance the tolerance to salt and high temperature stresses but reduce the tolerance to osmotic stress.

关 键 词：核黄素 水稻 非生物胁迫 幼苗生长 

分 类 号：Q945.78[生物学—植物学] S511[农业科学—作物学]