盐肤木体细胞胚胎发生及植株再生  

Somatic Embryogenesis and Plant Regeneration of Rhus chinen

作　　者：杨庆敏 尹飞飞 龚清 杨洋 桑子阳 刘文 YANG Qingmin;YIN Feifei;GONG Qing;YANG Yang;SANG Ziyang;LIU Wen(Key Laboratory of Three Gorges Regional Plant Genetics&Germplasm Enhancement/Biotechnology Research Center,School of Biology and Pharmacy,Yichang,Hubei 443002,China;Wufeng Boling Seed Industry Co.Ltd,Wufeng,Hubei 443413,China)

机构地区：[1]三峡区域植物遗传与种质创新重点实验室[三峡大学]/三峡大学生物技术研究中心,生物与制药学院,湖北宜昌443002 [2]五峰博翎种业有限公司,湖北五峰443413

出　　处：《西北植物学报》2023年第8期1369-1377,共9页Acta Botanica Boreali-Occidentalia Sinica

基　　金：宜昌市科技研究与开发项目(A21-4-013);五峰土家族自治县林业科学研究所委托项目(SDHZ2021081)。

摘　　要：以盐肤木(Rhus chinensis Mill.)幼胚为外植体,研究不同植物生长调节剂组合对其愈伤组织诱导及体细胞胚胎发生的影响,以建立盐肤木体细胞胚胎发生及植株再生体系。结果表明,最适愈伤组织诱导培养基为MS+6-BA 0.2 mg/L+2,4-D 1.0 mg/L,诱导率为84.57%,诱导出的初代愈伤组织白色或淡黄色,质地疏松,表面光滑,为非胚性愈伤。初代愈伤组织转移到1/2 MS+6-BA 2 mg/L+NAA 0.5 mg/L培养基上培养1个月后,长出淡黄色质地紧密的胚性愈伤组织,诱导率高达100%,在此培养基上胚性愈伤组织增殖倍数为854.73%。所获得的胚性愈伤组织转接到1/2 MS+6-BA 2 mg/L+NAA 0.5 mg/L+蔗糖4%的培养基上培养1个月后可诱导体细胞胚胎发生,诱导率可达32.67%。诱导得到的体细胞胚胎经历球形胚、心形胚、鱼雷胚、子叶胚进一步分化发育成苗。无菌苗炼苗后栽种到泥炭土∶蛭石∶珍珠岩为2∶1∶1的生长基质上,能100%稳定成活。经过细胞学观察分析,体细胞胚的发育与合子胚相似。In this study,immature embryos of Rhus chinensis were used as explants.In order to establish the system of somatic embryogenesis and plant regeneration,we studied the effects of different plant growth regulators on callus induction and somatic embryogenesis.The results showed that the optimal medium for callus induction was MS+6-BA 0.2 mg/L+2,4-D 1.0 mg/L,with an induction rate of 84.57%.The newly induced callus was white or light yellow,with loose texture and smooth surface.When the callus was transferred to 1/2 MS+6-BA 2 mg/L+NAA 0.5 mg/L medium for one month,the embryogenic callus with light yellow texture grew,and the induction rate was up to 100%,the multiplication of embryogenic callus on this medium was 854.73%.The embryogenic callus were transferred to 1/2 MS+6-BA 2 mg/L+NAA 0.5 mg/L+sucrose 4%medium for one month to induce somatic embryogenesis with an induction rate of 32.67%.The somatic embryos developed into plantlets through globular embryo,heart-shaped embryo,torpedo embryo and cotyledon embryo.Sterile seedlings can be planted to the peat∶vermiculite∶perlite 2∶1∶1 growth matrix,survival rate of 100%.The development of somatic embryos was similar to that of zygotic embryos.

关 键 词：盐肤木 愈伤组织 体细胞胚胎 细胞学观察 

分 类 号：S722.37[农业科学—林木遗传育种]