Ⅱ组和Ⅲ组mGluRs在慢性间歇性低氧诱导的大鼠颈动脉体可塑性中的作用  

Role of group Ⅱ and Ⅲ mGluRs in carotid body plasticity induced by chronic intermittent hypoxia in rats

作　　者：赵晨露 李超红[1] 刘玉珍[1] ZHAO Chen-Lu;LI Chao-Hong;LIU Yu-Zhen(The First Affiliated Hospital of Xinxiang Medical University,Henan Key Laboratory of Neural Repairment,Life Sciences Research Center,Weihui 453100,China)

机构地区：[1]新乡医学院第一附属医院,河南省神经修复重点实验室,生命科学研究中心,卫辉453100

出　　处：《生理学报》2023年第4期529-536,共8页Acta Physiologica Sinica

基　　金：supported by the National Natural Science Foundation of China (No. 81973988);Henan Key Laboratory of Neurorestoratology Foundation (No. HNSJXF-2021-014);the Graduate Innovation Program of Xinxiang Medical University (No. 202122Z)。

摘　　要：本文旨在探究Ⅱ组和Ⅲ组代谢型谷氨酸受体(metabotropic glutamate receptors, mGluRs)在慢性间歇性低氧(chronic intermittent hypoxia, CIH)所致大鼠颈动脉体可塑性中的作用。用Oxycycler A84特制低氧室对Sprague Dawley (SD)大鼠进行CIH处理4周,模型制备结束后测量大鼠尾动脉血压,用RT-qPCR检测颈动脉体mGluR2/3/8 mRNA表达水平,用离体胞外颈动脉窦神经放电记录技术检测颈动脉窦神经活性,并用急性间歇性低氧(acute intermittent hypoxia, AIH)诱导颈动脉体感觉长时程易化(sensory long-term facilitation, sLTF),观察Ⅱ组和Ⅲ组mGluRs在CIH诱导的颈动脉体可塑性中的作用。结果显示,CIH处理4周后,和对照组相比,CIH组大鼠血压明显升高,颈动脉体mGluR2/3 mRNA表达水平下调,而mGluR8 mRNA表达水平上调;电生理结果显示,AIH诱导CIH组颈动脉体出现sLTF,激活Ⅱ组mGluRs对CIH组大鼠颈动脉体sLTF没有影响,而激活Ⅲ组m Glu Rs完全抑制s LTF。以上结果提示,CIH可导致大鼠血压升高,且激活Ⅲ组m Glu Rs可抑制CIH诱导的颈动脉体可塑性。The aim of the present study was to explore the role of group Ⅱ and Ⅲ metabotropic glutamate receptors(mGluRs) in carotid body plasticity induced by chronic intermittent hypoxia(CIH) in rats. Sprague Dawley(SD) rats were treated with CIH in Oxycycler A84 hypoxic chamber for 4 weeks, and the tail artery blood pressure was measured at the end of model preparation. RT-qPCR was performed to examine the mRNA expression levels of mGluR2/3/8 in rat carotid body. Carotid sinus nerve activity was detected by ex vivo carotid sinus nerve discharge recording technique, and acute intermittent hypoxia(AIH) was administered to induce carotid body sensory long-term facilitation(sLTF), in order to observe the role of group Ⅱ and group Ⅲ mGluRs in carotid body plasticity induced by CIH. The results showed that: 1) After 4 weeks of CIH exposure, the blood pressure of rats increased significantly;2) CIH down-regulated the mRNA levels of mGluR2/3, and up-regulated the mRNA level of mGluR8 in the carotid body;3) AIH induced sLTF in carotid body of CIH group. In the CIH group, activation of group Ⅱ mGluRs had no effect on sLTF of carotid body, while activation of group Ⅲ mGluRs completely inhibited sLTF. These results suggest that CIH increases blood pressure in rats, and group Ⅲ mGluRs play an inhibitory role in CIH-induced carotid body plasticity in rats.

关 键 词：Ⅱ组mGluRs Ⅲ组mGluRs 颈动脉体 可塑性 

分 类 号：R766[医药卫生—耳鼻咽喉科]