广西沃柑根系内生真菌的多样性分析  

Diversity of endophytic fungi in Orah mandarin roots in Guangxi

作　　者：徐杰 温云英 莫惠捷 康贻豪 陈廷速[2] 张金莲[2] 姜明国 XU Jie;WEN Yun-ying;MO Hui-jie;KANG Yi-hao;CHEN Ting-su;ZHANG Jin-lian;JIANG Ming-guo(School of Marine Sciences and Biotechnology,Guangxi University for Nationalities,Nanning,Guangxi 530008,China;Institute of Microbiology,Guangxi Academy of Agricultural Sciences,Nanning,Guangxi 530007,China)

机构地区：[1]广西民族大学海洋与生物技术学院,广西南宁530008 [2]广西农业科学院微生物研究所,广西南宁530007

出　　处：《南方农业学报》2023年第6期1800-1809,共10页Journal of Southern Agriculture

基　　金：广西科技重大专项(桂科AA18242026);广西科技基地和人才专项(桂科AD20159001);厦门大学联合项目(KJ2023480B);广西农业科学院基本科研业务专项(2023YM96)。

摘　　要：【目的】探究清耕和生草措施对沃柑根系内生真菌多样性的影响,为筛选和开发促生、防病和抗逆等功能的有益内生真菌资源提供科学依据。【方法】选取广西南宁市武鸣区6个代表性沃柑果园(生草模式和清耕模式各3个),调查其土壤养分水平,利用高通量测序技术对沃柑根系内生真菌ITS区进行测定,分析沃柑根系样品中真菌群落的多样性及其分布规律。【结果】广西南宁市武鸣区沃柑果园的土壤为酸性,速效钾、有机质含量丰富。从沃柑果树根系样品中共检测出内生真菌12门42纲102目224科416属,其中真菌在门分类水平上的主要优势类群是子囊菌门(Ascomycota),在属分类水平上的主要优势类群为新赤壳属(Neocosmospora)和青霉属(Penicillium),自然生草及清耕模式下的沃柑根系内生真菌群落结构无明显差异。典型相关分析(CCA)结果表明,沃柑果园根系真菌群落与硝态氮、铵态氮、土壤有机质呈极显著相关(P<0.01),与pH呈显著相关(P<0.05),与速效磷、速效钾无显著相关性(P>0.05)。所有试验果园根系中的营养功能类群结构均相似,以腐生-营养型内生真菌菌群的相对丰度最高,其次为未鉴定的功能群、病理-腐生-共生营养型,其他营养功能类群在不同果园中占比均较低。【结论】广西沃柑根系内生真菌以新赤壳属和青霉属为优势属,该区域尺度下的沃柑根系内生真菌群落组成与生草管理无显著相关,但受部分土壤环境因子调控。【Objective】This paper investigated the effects of clean tillage and grassing measures on the diversity of endophytic fungi in Orah mandarin roots,to provide a scientific basis for screening and developing beneficial endophytic fungal resources with functions such as growth promotion,disease prevention and stress resistance.【Method】Six representative Orah mandarin orchards in Wuming District,Nanning City,Guangxi(three grassing mode and three clean tillage mode)were selected to investigate their soil nutrient levels.And the ITS zones of endophytic fungi in Orah mandarin roots were determined using high-throughput sequencing technology to analyze the diversity of fungal communities in Orah mandarin root samples and their distribution patterns.【Result】The soil of Orah mandarin orchards in Wuming District,Nanning,Guangxi was mainly acidic,being rich in available potassium and organic matter.Endophytic fungi were detected in endophytic fungi in Orah mandarin root samples,including 12 phylums,42 classes,102 orders,224 families and 416 genera of endophytic fungi,of which the main dominant taxa at the phylum level was Ascomycota,and at the genus level were Neocosmospora,Penicillium.There was no great difference in the community structure of endophytic fungi in Orah mandarin roots under natural grassing and clean tillage modes.The results of canonical correlation analysis(CCA)showed that the fungal communities in Orah mandarin root were highly significantly correlated with nitrate nitrogen,ammonium nitrogen and soil organic matter(P<0.01),significantly correlated with pH(P<0.05),and not significantly correlated with available phosphorus and available potassium(P>0.05).The structures of nutrient functional groups in the root system were similar in all the experimental orchards,with the highest relative abundance of saprophytic-nutrient endophytic fungal communities,followed by unidentified functional groups,pathological-saprophytic-symbiotic nutrient fungal communities.And the other nutrient functional groups oc

关 键 词：沃柑 植物内生真菌 群落多样性 FUNGuild 广西 

分 类 号：S666.1[农业科学—果树学]