基于超高效液相色谱–质谱联用技术鉴别血迹组织来源  

Identifi cation of the Tissue Source of Blood Stains Based on Ultra-High Performance Liquid Chromatography-Mass Spectrometry

作　　者：石慧霞 唐朝 胡灿 郭洪玲 孙启凡 罗群[3,4] 季安全 朱军 SHI Huixia;TANG Chao;HU Can;GUO Hongling;SUN Qifan;LUO Qun;JI Anquan;ZHU Jun(Institute of Forensic Science,Ministry of Public Security,Beijing 100038,China;National Centre for Mass Spectrometry in Beijing,Beijing 100190,China;Beijing National Laboratory for Molecular Sciences,Key Laboratory of Analytical Chemistry for Living Biosystems,Institute of Chemistry,Chinese Academy of Sciences,Beijing 100190,China;University of Chinese Academy of Sciences,Beijing 100049,China)

机构地区：[1]公安部鉴定中心,北京100038 [2]北京质谱中心,北京100190 [3]北京分子科学国家实验室,中国科学院化学研究所活体分析化学实验室,北京100190 [4]中国科学院大学,北京100049

出　　处：《刑事技术》2023年第5期473-480,共8页Forensic Science and Technology

基　　金：中央级公益性科研院所基本科研业务费专项资金项目(2019JB022)。

摘　　要：犯罪现场血液斑迹的成分蕴含许多与案件相关的重要信息,通过血迹成分检测,推断血迹组织来源,有助于案件性质的分析研判和法庭诉讼的技术支撑。基于不同体液的生理功能和体内代谢的显著差异,不同组织来源的血液中差异稳定的内源性小分子可作为鉴别血迹组织来源的重要依据。本文应用超高效液相色谱–质谱联用技术(UHPLC-MS),以小分子化合物为分析对象,通过对外周血、月经血实验样品的前处理、液相分离富集及高分辨质谱检测,综合多种数据分析手段,筛选出区分外周血与月经血的特征小分子,并结合人类代谢组学数据库(HMDB)进行结构及性能检索,确定外周血7个特征小分子和月经血4个特征小分子。将建立的基于UHPLC-MS的血迹组织来源鉴别方法应用于10份血迹样品的测试,鉴别结果准确。本文建立的方法可为实际案件中血迹组织来源鉴别提供新的技术手段。The composition of blood stains at the crime scene contains important information in many cases.Through the detection of blood components and the inference of the source of blood stains,it is helpful to the analysis and judgment of the nature of the case and the technical support of court proceedings.Based on the biological function of different tissues and the differences in biological metabolism,the stable endogenous small molecules in blood from different tissues can be an important basis for identifying the origin of blood tissues.In this paper,ultra-high performance liquid chromatography and mass spectrometry(UHPLC-MS)were used to analyze small molecule compounds.Through the pre-treatment of peripheral blood and menstrual blood samples,liquid separation and enrichment,and high resolution mass spectrometry,a variety of data analysis methods were integrated to screen out characteristic small molecules that distinguish peripheral blood and menstrual blood.Seven characteristic small molecules in peripheral blood and four characteristic small molecules in menstrual blood were identifi ed combining with the human metabolome database(HMDB).The established UHPLC-MS method was applied to the detection of 10 blood samples,and the accurate identifi cation results were obtained.Therefore,the method established in this paper can provide a new technical means for the identifi cation of blood tissue sources in actual cases.

关 键 词：超高效液相色谱–质谱联用技术 血液斑迹 外周血 月经血 特征小分子 

分 类 号：O657.63[理学—分析化学] D918.9[理学—化学]