彝族高血压患者代谢产物分析及相关机制研究  

Analysis of metabolites and related mechanisms of hypertensive patients in Yunnan Yi nationality area

作　　者：李文俊 姬燕梅 杨云红[1] 田海涛 孙嫄 周丹 李吉胜 郭妮 蒙妮 金醒昉[1] LI Wenjun;JI Yanmei;YANG Yunhong;TIAN Haitao;SUN Yuan;ZHOU Dan;LI Jisheng;GUO Ni;MENG Ni;JIN Xingfang(Department of Cardiovascular Surgery,Affiliated Yan’an Hospital,Kunming Medical University,Kunming,Yunnan 650051,China)

机构地区：[1]昆明医科大学附属延安医院心脏大血管外科,昆明650051

出　　处：《重庆医学》2023年第19期2912-2918,共7页Chongqing medicine

基　　金：国家自然科学基金项目(81460209);云南省科技厅中央引导地方发展资金项目(202307AB110005,202007AA110013);云南省肿瘤免疫防治重点实验室项目(2017DG004-01);云南省昆明市科技局计划项目(2019-1-N-25318000002911)。

摘　　要：目的探讨彝族原发性高血压与代谢产物间的关系及相关机制。方法2018年11月至2020年10月采用超高效液相色谱-飞行时间串联质谱(UHPLC-QE-MS)分析彝族原发性高血压患者20例(高血压组)与健康者10例(对照组)的血清代谢产物。寻找与云南彝族地区原发性高血压相关的差异代谢物及代谢通路,获得云南彝族地区原发性高血压的相关代谢标志物。结果筛选出彝族高血压患者的4种血清代谢产物相对水平明显升高,包括邻苯二酚、马尿酸、N-乙酰芳基胺、辛弗林丙酮,差异有统计学意义(P<0.05)。与对照组比较,彝族高血压患者的差异代谢通路包括甘油磷脂代谢、鞘脂代谢、苯丙氨酸代谢、丙氨酸、天冬氨酸和谷氨酸代谢通路。结论4种差异代谢产物和4条差异代谢通路与彝族患者原发性高血压的发生、发展密切相关。Objective To explore the relationship between essential hypertension and metabolites in Yi nationality and its related mechanism.Methods The serum metabolites of 20 patients with essential hypertension(the hypertension group)and 10 healthy persons(the control group)in the Yi nationality area of Yunnan were analyzed by ultra-high performance liquid chromatography-time-of-flight tandem mass spectrometry(UHPLC-QE-MS)from November 2018 to October 2020.The differential metabolites and metabolic pathways related to essential hypertension in Yi nationality were found,and the metabolic markers related to essential hypertension in Yunnan Yi area were obtained.Results The relative contents of four serum metabolites in Yi nationality patients with hypertension were screened to be significantly increased,including Pyrocatechol,Hippuric acid,N-Acetylarylamine,Synephrine acetonide,the difference was statistically significant(P<0.05).Compared with the control group,the differential metabolic pathways of Yi hypertensive patients included glycerophospholipid metabolism,sphingolipid metabolism,phenylalanine metabolism,alanine,aspartic acid and glutamic acid metabolism.Conclusion The above four differential metabolites and four differential metabolic pathways are closely related to the occurrence and development of essential hypertension in Yi nationality.

关 键 词：高血压 代谢产物 代谢组学 彝族 机制 

分 类 号：R544.1[医药卫生—心血管疾病]