罗汉松幼苗在不同磷环境下添加氮的生理响应  

Physiological Response of Podocarpus macrophyllus Seedlings to Nitrogen Addition in Different Phosphorus Environments

作　　者：权佳惠 招礼军[1] 朱栗琼[1] 邱广龙[2] QUAN Jiahui;ZHAO Lijun;ZHU Liqiong;QIU Guanglong(Guangxi Key Laboratory of Forest Ecology and Conservation/Forestry College,Guangxi University,Nanning,Guangxi 530004,China;Guangxi Mangrove Research Center,Guangxi Academy of Sciences,Beihai,Guangxi 536000,China)

机构地区：[1]广西大学林学院/广西森林生态与保育重点实验室,广西南宁530004 [2]广西科学院广西红树林研究中心,广西北海536000

出　　处：《热带作物学报》2023年第9期1817-1828,共12页Chinese Journal of Tropical Crops

基　　金：国家自然科学基金项目(No.31560061);广西自然科学基金项目(No.2013GXNSFAA019063)。

摘　　要：罗汉松(Podocarpus macrophyllus)是我国南方地区常见的庭院观赏和绿化树种,具有较高的经济和药用价值。为探究罗汉松幼苗在不同磷环境下添加氮的生理响应,以2年生罗汉松幼苗为研究试材,设置低磷(0.26 g/株)、高磷(1.08 g/株)以及低氮(0.53 g/株)、中氮(1.06 g/株)和高氮(2.12 g/株)处理,分析各处理组罗汉松幼苗生理特性、叶绿素含量、气体交换参数和叶片养分含量的差异。结果表明:(1)氮添加提高了罗汉松幼苗过氧化物酶(POD)活性,高浓度氮肥降低了超氧化物歧化酶(SOD)活性;低磷环境施加氮肥,罗汉松幼苗可溶性糖和可溶性蛋白含量增加,丙二醛(MDA)和游离脯氨酸(Pro)含量下降;高磷环境高氮浓度下,可溶性蛋白和可溶性糖含量积累量减少,MDA和Pro含量增加。(2)中浓度氮肥处理组罗汉松幼苗叶绿素含量最高;低磷环境氮添加提高了罗汉松幼苗叶片净光合速率(P_(n))、蒸腾速率(T_(r))和气孔导度(G_(s)),罗汉松幼苗胞间CO_(2)浓度(C_(i))降低,高磷环境中浓度氮肥处理组罗汉松幼苗P_(n)、T_(r)和G_(s)最高,Ci最低,高磷高氮下P_(n)、T_(r)和G_(s)降低,Ci含量增加;P_(n)和T_(r)相同的变化趋势以及相关性分析表明,罗汉松幼苗P_(n)主要受气孔因素限制。(3)氮添加促进了低磷环境下罗汉松幼苗叶片氮含量增加,但降低了高磷环境下叶片氮含量的增加;氮添加限制了罗汉松幼苗叶片对磷的积累,但土壤磷素的增加促进了叶片磷含量积累。(4)磷肥浓度增加提高了不同氮处理下罗汉松幼苗酶活性;增加氮肥浓度降低了低氮和高氮处理组MDA和Pro含量,可溶性糖和可溶性蛋白含量增加;高氮浓度下磷肥浓度增加,罗汉松MDA和Pro含量增加,可溶性糖和可溶性蛋白含量下降;增加磷肥浓度促进了低氮和中氮处理组罗汉松幼苗叶绿素含量,提升其光合效率;而高氮浓度下增加磷肥含量,罗汉松幼苗叶绿素和光合效率降低Podocarpus macrophyllus is a common garden ornamental and greening tree species in southern China,with high economic and medicinal value.To study the physiological response of nitrogen addition under different phosphorus environments to P.macrophyllus seedlings,two-year-old P.macrophyllus seedlings were used as the material,two soil phosphorus conditions including low additional phosphorous(0.26 g/plant),high phosphorus addition(1.08 g/plant),three nitrogen addition levels including low nitrogen addition(0.53 g/plant),medium nitrogen addition(1.06 g/plant)and high nitrogen addition(2.12 g/plant)were set up and analyzed the differences of physiological characteristics,chlorophyll content,gas exchange parameters and leaf nutrient content of P.macrophyllus seedlings in each treatment group.Nitrogen addition increased the activity of POD,and high concentration nitrogen fertilizer decreased the activity of SOD.The application of nitrogen fertilizer in a low phosphorus environment increased soluble sugars and soluble proteins,decreased MDA and Pro.Soluble protein and soluble sugar accumulation decreased in high nitrogen and phosphorus environment,but MDA and Pro increased.The chlorophyll content of P.macrophyllus seedlings was the highest under medium concentration nitrogen treatment.The addition of nitrogen in low phosphorus environment in-creased the P_(n),T_(r)and G_(s)of P.macrophyllus seedlings,while C_(i)(intercellular CO_(2)concentration)of P.macrophyllus seedlings decreased.The P_(n),T_(r)and G_(s)were the highest and C_(i)was the lowest in the medium nitrogen treatment group in high phosphorus environment.P_(n),T_(r)and G_(s)decreased,and the C_(i)increased in the high nitrogen concentration and high phosphorus environment.The same change trend of P_(n)and T_(r)and correlation analysis showed that P_(n)of P.macrophyllus seedlings was mainly limited by stoma factors.Nitrogen addition promoted the increase of leaf nitrogen content in low phosphorus environment,but decreased the leaf nitrogen content in high phos

关 键 词：罗汉松 幼苗 氮 磷 生理响应 光合作用 养分 

分 类 号：S791.46[农业科学—林木遗传育种]