叶面喷施羧基化碳纳米管对平邑甜茶幼苗生长及抗氧化系统的影响  

Effects of Foliar Application of Carboxylated Carbon Nanotubes on Growth and Antioxidant System of Malus hupehensis

作　　者：吴康 苏安琪 田雪娇 李传收 付志顺 杨静静 宿红艳 Wu Kang;Su Anqi;Tian Xuejiao;Li Chuanshou;Fu Zhishun;Yang Jingjing;Su Hongyan(College of Agronomy,Ludong University,Yantai 264025,China)

机构地区：[1]鲁东大学农学院,山东烟台264025

出　　处：《山东农业科学》2023年第9期121-127,共7页Shandong Agricultural Sciences

基　　金：国家自然科学基金项目(32001988,32071788);山东省自然科学基金项目(ZR2021MC102,ZR2020MC153)。

摘　　要：为研究碳纳米管(carbon nanotubes,CNTs)对植物生长发育的影响,本试验以苹果砧木平邑甜茶(Malus hupehensis)为材料,设置叶面喷施浓度为0、10 mg/L和20 mg/L羧基化单壁碳纳米管(SWCNTs)处理,研究其对幼苗地上部生长和叶片光合作用、抗氧化系统等生理生化指标的影响。结果表明,叶面喷施10、20 mg/L SWCNTs均能显著促进平邑甜茶幼苗地上部生长和叶片光合作用,有效增强叶片过氧化物酶(POD)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)、谷胱甘肽还原酶(GR)和脱氢抗坏血酸还原酶(DHAR)活性,提高抗坏血酸(AsA)和谷胱甘肽(GSH)含量。与对照相比,喷施10、20 mg/L SWCNTs处理叶片脱氢抗坏血酸(DHA)和氧化型谷胱甘肽(GSSG)含量显著降低,而过氧化氢(H_(2)O_(2))和丙二醛(MDA)含量没有显著变化。透射电镜结果显示,SWCNTs能穿透并聚集在叶片细胞内部。综上,叶面喷施10、20 mg/L SWCNTs能促进平邑甜茶地上部生长,提高叶片叶绿素含量和光合作用,上调抗氧化酶活性,并加快AsA-GSH循环系统的高效运转,使细胞保持较高的抗氧化能力。In order to study the effect of carbon nanotubes(CNTs)on plant growth and development,the apple rootstock Malus hupehensis was used as experimental material,and the foliar application of 0,10 and 20 mg/L carboxylated single-walled carbon nanotubes(SWCNTs)were set to study their effects on the aboveground growth of seedlings,leaf photosynthesis,antioxidant system and other physiological and biochemical indexes.The results showed that spraying 10 and 20 mg/L SWCNTs significantly promoted shoot growth and leaf photosynthesis,effectively enhanced the activities of peroxidase(POD),superoxide dismutase(SOD),catalase(CAT),ascorbate peroxidase(APX),glutathione reductase(GR)and dehydroascorbate reductase(DHAR)in leaves,and increased the contents of ascorbic acid(AsA)and glutathione(GSH).Compared with the control,the contents of dehydroascorbic acid(DHA)and oxidized glutathione(GSSG)in leaves sprayed with 10 and 20 mg/L SWCNTs decreased significantly,while the contents of hydrogen peroxide(H_(2)O_(2))and malondialdehyde(MDA)did not change significantly.The results of TEM detection showed that SWCNTs particles could penetrate and aggregate inside leaf cells.In conclusion,foliar application of 10 and 20 mg/L SWCNTs could promote the growth of M.hupehensis shoots,improve the chlorophyll and photosynthesis content of leaves,enhance the activities of antioxidant enzymes(POD,SOD and CAT),accelerate the efficient operation of ASA-GSH circulation system,and maintain the high antioxidant capacity of cells.

关 键 词：碳纳米管 平邑甜茶 叶片 抗氧化系统 光合作用 

分 类 号：S661.401[农业科学—果树学]