荨麻叶的化学成分研究  

Studies on chemical constituents of Urtica fissa leaves

作　　者：王晨曦 王作强 李育晓 白溪山[1] 陈林[1] 黄相中[1] 李艳红[1] WANG Chen-xi;WANG Zuo-qiang;LI Yu-xiao;BAI Xi-shan;CHEN Lin;HUANG Xiang-zhong;LI Yan-hong(Key Laboratory of Chemistry in Ethnic Medicinal Resouces,State Ethnic Affairs Commission and Ministry of Education,Yunnan Minzu University,Kunming 650500,China)

机构地区：[1]云南民族大学民族药资源化学国家民委-教育部重点实验室,云南昆明650500

出　　处：《云南民族大学学报（自然科学版）》2023年第5期541-546,共6页Journal of Yunnan Minzu University:Natural Sciences Edition

基　　金：国家自然科学基金(81960783);云南省重大科技专项(生物医药)(202102AA100018);云南省自然科学基金(202101AT070111)。

摘　　要：综合运用硅胶柱层析、凝胶柱层析和高效液相色谱等多种现代分离的方法,对荨麻叶的体积分数为95%乙醇提取物进行化学成分研究,共分离得到10个化合物.根据其理化性质、核磁数据及对照标准品鉴定了化合物的结构,分别为:(13^(2))-羟基脱镁叶绿酸a甲酯(1)、葫芦巴碱(2)、甜菜碱(3)、黑麦草内酯(4)、对羟基苯甲醛(5)、香草醛(6)、对羟基苯乙酸甲酯(7)、蔗糖(8)、山奈酚-3-O-葡萄糖苷(9)、β-谷甾醇(10).其中化合物1和2为首次从荨麻中获得.采用脂多糖(LPS)诱导小鼠单核巨噬细胞(RAW264.7)释放NO的炎症模型测试了化合物1~7及化合物9的抗炎活性.The 95%ethanol extract of Urtica fissa leaves was investigated by using modern separation methods such as silica gel,Sephadex LH-20 chromatography and high performance liquid chromatography.Ten compounds were isolated and identified as(13^(2))-hydroxypheophorbide a methyl ester(1),trigonelline(2),betaine(3),loliolide(4),p-hydroxybenzaldehyde(5),vanillin(6),4-hydroxyphenylacetic acid methyl ester(7),sucrose(8),kaempferol 3-O-glucoside(9),andβ-sitosterol(10).according to their physical and chemical properties,NMR spectral data and control standards.Compounds 1 and 2are isolated from this plant for the first time.The anti-in⁃flammatory activity of some compounds was measured by LPS induction to release NO from RAW264.7.

关 键 词：荨麻 化学成分 生物碱 抗炎 

分 类 号：R284.1[医药卫生—中药学]