基于网络药理学探究石斛碱治疗阿尔兹海默病的关键靶点  

To Explore the Key Targets of Dendrobine in the Treatment of Alzheimer’s Disease Based on Network Pharmacology

作　　者：洪梦杰 于白音 刘洋洋 陈娟 陈洁 HONG Mengjie;YU Baiyin;LIU Yangyang;CHEN Juan;CHEN Jie(Medical College,Henan Vocational University of Science and Technology,Zhoukou 466000,Henan,China;Guangdong Provincial key Laboratory of Utilization and Conservation of Food and Medicinal Resources in Northern Region,Shaoguan University,Shaoguan 512005,Guangdong,China;Xing’an League Inspection and Testing Center for Food and Drug Control,Ulanhot 137400,Inner Mongolia,China)

机构地区：[1]河南科技职业大学医学院,河南周口466000 [2]韶关学院广东省粤北食药资源利用与保护重点实验室,广东韶关512005 [3]兴安盟食品药品检验检测中心,内蒙古乌兰浩特137400

出　　处：《韶关学院学报》2023年第9期51-55,共5页Journal of Shaoguan University

基　　金：韶关学院重点项目“铁皮石斛品种选育研究”(SZ2022KJ04);广东省教育厅普通高校重点项目“丹霞铁皮石斛黄酮类化合物研究”(2021ZDX4059)。

摘　　要：基于网络药理学初步探寻石斛碱对阿尔兹海默病作用的关键靶点.通过Swiss Target Prediction数据库收集石斛碱靶点,通过Gene Cards数据库收集疾病靶点,利用STRING 11.5数据平台获得药物和疾病交集基因的PPI数据,将结果导入Cytoscape 3.9.1软件,拓扑分析得到核心靶点,利用DAVID数据库对核心靶点进行GO功能富集和KEGG通路富集分析,建立“石斛碱-核心靶点-通路”网络图,筛选得到关键靶点.研究共获得石斛碱靶点139个,疾病靶点9691个,共有靶点125个;经过蛋白互作及拓扑分析,得到核心靶点24个,核心靶点涉及140个基因功能条目和27条通路(P<0.05);将“石斛碱-核心靶点-通路”网络图进行拓扑分析,共筛选出GRIN2B、GRIN2A、GRIN1、PSMD2、PSMC1、PSMC4、PSMD14、PSMC6和DRD2共9个关键靶点.石斛碱可能通过调节这些关键靶点来实现对阿尔兹海默病的治疗,为后续石斛碱治疗阿尔兹海默病相关机制的研究提供思路和依据.Based on network pharmacology,the key targets of dendrobine on Alzheimer’s disease were preliminarily explored.The targets of dendrobine were collected by Swiss Target Prediction database,and the disease targets were collected by GeneCards database.The PPI data of drug and disease intersection genes were obtained by STRING 11.5 data platform.The results were imported into Cytoscape 3.9.1 software,and the core targets were obtained by topological analysis.The DAVID database was used to analyze the GO function enrichment and KEGG pathway enrichment of the core targets,and the“dendrobine-core target-pathway”network diagram was established to screen the key targets.A total of 139 dendrobine targets,9691 disease targets and 125 common targets were obtained.After protein interaction and topology analysis,24 core targets were obtained,which involved 140 gene function entries and 27 pathways(P<0.05).The“dendrobine-core target-pathway”network diagram was topologically analyzed,and a total of 9 key targets including GRIN2B,GRIN2A,GRIN1,PSMD2,PSMC1,PSMC4,PSMD14,PSMC6 and DRD2 were screened out.The study found that dendrobine has the characteristics of multi-target and multi-pathway in the treatment of Alzheimer’s disease.The therapeutic effect of dendrobine on Alzheimer’s disease may be achieved by regulating these targets,which provides ideas and basis for subsequent research on the mechanism of dendrobine in the treatment of Alzheimer’sdisease.

关 键 词：金钗石斛 石斛碱 阿尔兹海默病 治疗 靶点 网络药理学 

分 类 号：R285[医药卫生—中药学]