Heparinized PGA host-guest hydrogel loaded with paracrine products from electrically stimulated adipose-derived mesenchymal stem cells for enhanced wound repair  

作　　者：Mengyao Li Junliang Li Xueru Xiong Yushi Wang Yong-Guang Jia Xuetao Shi Xiaoling Fu 

机构地区：[1]School of Materials Science and Engineering,South China University of Technology,Guangzhou 510641,China [2]School of Biomedical Sciences and Engineering,South China University of Technology,Guangzhou International Campus,Guangzhou 511442,China [3]National Engineering Research Center for Tissue Restoration and Reconstruction and Innovation Center for Tissue Restoration and Reconstruction,Guangzhou 510006,China [4]Key Laboratory of Biomedical Engineering of Guangdong Province,South China University of Technology,Guangzhou 510006,China

出　　处：《Engineered Regeneration》2023年第3期225-237,共13页再生工程（英文）

基　　金：supported by the National Natu-ral Science Foundation of China (T2288101,31971266,82272152,22075087);Guangdong Basic and Applied Basic Research Foundation (2022A1515011925);the Key Research and Development Program of Guangzhou (202007020002).

摘　　要：The microenvironment of the wound bed is essential in the regulation of wound repair.In this regard,strategies that provide a repairing favorable microenvironment may effectively improve healing outcomes.Herein,we attempted to use electrical stimulation(ES)to boost the paracrine function of adipose-derived stem cells from rats(rASCs).By examining the concentrations of two important growth factors,VEGF and PDGF-AA,in the cell culture supernatant,we found that ES,especially 5𝜇A ES,stimulated rASCs to produce more paracrine factors(5𝜇A-PFs).Further studies showed that ES may modulate the paracrine properties of rASCs by upregulating the levels of TRPV2 and TRPV3,thereby inducing intracellular Ca^(2+) influx.To deliver the PFs to the wound to effectively improve the wound microenvironment,we prepared a heparinized PGA host-guest hydrogel(PGA-Hp hydrogel).Moreover,PGA-Hp hydrogel loaded with 5𝜇A-PFs effectively accelerated the repair process of the full-thickness wound model in rats.Our findings revealed the effects of ES on the paracrine properties of rASCs and highlighted the potential application of heparinized PGA host-guest hydrogels loaded with PFs derived from electrically stimulated rASCs in wound repair.

关 键 词：Wound repair Electrical stimulation Paracrine factors Heparinized PGA host-guest hydrogel 

分 类 号：R318.08[医药卫生—生物医学工程]