The pyruvate dehydrogenase kinase 2(PDK2)is associated with conidiation,mycelial growth,and pathogenicity in Fusarium graminearum  

作　　者：Tao Gao Dan He Xin Liu Fang Ji Jianhong Xu Jianrong Shi 

机构地区：[1]Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base,Ministry of Science and Technology/Key Laboratory for Control Technology and Standard for Agro-product Safety and Quality,Ministry of Agriculture and Rural Affairs/Key Laboratory for Agro-product Safety Risk Evaluation(Nanjing),Ministry of Agriculture and Rural Affairs/Collaborative Innovation Center for Modern Grain Circulation and Safety/Institute of Food Safety and Nutrition,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,Jiangsu,China [2]School of Food and Biological Engineering,Jiangsu University,Zhenjiang,Jiangsu,China

出　　处：《Food Production, Processing and Nutrition》2020年第1期102-111,共10页食物生产加工与营养(英文)

基　　金：This work supported by the National Natural Science Foundation(31772118,31872914,31901936);Jiangsu Agriculture Science and Technology Innovation Fund(CX(19)3004,CX(17)1003).

摘　　要：Pyruvate dehydrogenase kinase(PDK)is a mitochondrial enzyme in a variety of eukaryotes,including the plant pathogen Fusarium graminearum.This enzyme can reduce the oxidation of glucose to acetyl-coA by phosphorylation and selectively inhibits the activity of pyruvate dehydrogenase(PDH),which is a kind of pyruvate dehydrogenase complex(PDC).In this study,we investigated the F.graminearum pyruvate dehydrogenase kinase encoded by FgPDK2,which is a homologue of Neurospora crassa PDK2.The disruption of the FgPDK2 gene led to several phenotypic defects including effects on mycelial growth,conidiation,pigmentation,and pathogenicity.The mutants also showed decreased resistance to osmotic stress and cell membrane/wall-damaging agents.The FgPDK2 deletion mutant exhibited reduced virulence.All of these defects were restored by genetic complementation of the mutant with the complete FgPDK2 gene.Overall,the results demonstrated that FgPDK2 is crucial for the growth of F.graminearum and can be exploited as a potential molecular target for novel fungicides to control Fusarium head blight caused by F.graminearum.

关 键 词：Fusarium graminearum Pyruvate dehydrogenase kinase Vegetative differentiation Reactive oxygen species(ROS) 

分 类 号：S43[农业科学—农业昆虫与害虫防治]