Classification of archaic rice grains excavated at the Mojiaoshan site within the Liangzhu site complex reveals an Indica and Japonica chloroplast complex  

作　　者：Katsunori Tanaka Chunfang Zhao Ningyuan Wang Shinji Kubota Masaaki Kanehara Nobuhiko Kamijo Ryuji Ishikawa Hiroyuki Tasaki Minako Kanehara Bin Liu Minghui Chen Shin-ichi Nakamura Tetsuro Udatsu Cailin Wang 

机构地区：[1]Faculty of Agriculture and Life Science,Hirosaki University,3 Bunkyo,Hirosaki,Aomori 036-8561,Japan [2]Institute of Food Crops,Jiangsu Academy of Agricultural Sciences,Xiaolingwei,Nanjing 210014,Jiangsu Province,China [3]Zhejiang Provincial Institute of Cultural Relics and Archaeology,Gongshuqu Jiashanxincun26,Hangzhou 310014,Zhejiang Province,China [4]Center for Cultural Resource Studies Kanazawa University,Kanazawa University,Kakuma,Kanazawa,Ishikawa 920-1192,Japan [5]Department of Teacher Training and School Education,Nara University of Education,Takahata,Nara,Nara 630-8528,Japan [6]Archaeological Research Center,Ehime University,10-13 Dogo-hi-mata,Matsuyama,Ehime 790-8577,Japan [7]Agricultural Museum,Faculty of Agriculture,Miyazaki University,1-1 Gakuen-Kibana-dai-Nishi,Miyazaki,Miyazaki 889-2192,Japan

出　　处：《Food Production, Processing and Nutrition》2020年第1期148-159,共12页食物生产加工与营养（英文）

基　　金：supported by a Scientific Research on Innovative Areas of the JSPS Grant-in-Aid(No.15H05968).

摘　　要：To understand rice types that were utilized during postdomestication and in the modern age and the potential of genetic research in aged rice materials,archaeogenetic analysis was conducted for two populations of archaic rice grains from the Mojiaoshan site during the Liangzhu Period in China(2940 to 2840 BC).Sequencing after the PCR amplification of three regions of the chloroplast genome and one region of the nuclear genome showed recovery rates that were comparable to those in previous studies except for one chloroplast genome region,suggesting that the materials used in this work were appropriate for recovering genetic information related to domestication traits by using advanced technology.Classification after sequencing in these regions proved the existence of Japonica and Indica chloroplasts in archaic grains from the west trench,which were subsequently classified into eight plastid groups(type I–VIII),and indicated that these rice grains derived from different maternal lineages were stored together in storage houses at the Mojiaohsan site.Among these plastid groups,type V exhibited the same sequences as two modern Indica accessions that are utilized in basic studies and rice breeding.It was inferred that part of the chloroplast genome of archaic rice has been preserved in modern genetic resources in these two modern Indica accessions,and the results indicated that rice related to their maternal ancestor was present at the Mojiaoshan site during the Liangzhu Period in China.The usefulness of archaeogenetic analysis can be demonstrated by our research data as well as previous studies,providing encouragement for the possibility that archaeogenetic analysis can be applied to older rice materials that were utilized in the rice-domesticated period.

关 键 词：Agriculture ARCHAEOLOGY Archaic DNA Diversity Mojiaoshan Rice 

分 类 号：X70[环境科学与工程—环境工程]