High-yield production of secoisolariciresinol diglucoside from flaxseed hull by extraction with alcoholic ammonium hydroxide and chromatography on microporous resin  

作　　者：Cong-Cong Zhuang Chun-Rui Liu Cheng-Bin Shan Zheng Liu Ling Liu Chao-Mei Ma 

机构地区：[1]State Key Laboratory of Reproductive Regulation&Breeding of Grassland Livestock,Hohhot,China [2]Key Laboratory of Herbage&Endemic Crop Biology of Ministry of Education,School of Life Sciences,Inner Mongolia University,Hohhot,P.R.China 010070 [3]Inner Mongolia Yishanyuan Biotechnology Co.,Ltd,Hohhot,People’s Republic of China

出　　处：《Food Production, Processing and Nutrition》2021年第1期450-459,共10页食物生产加工与营养（英文）

基　　金：supported by the Science and Technology Program of Inner Mongolia Autonomous Region of China to Chao-Mei Ma(2019GG247);the science and technology major project of Inner Mongolia Autonomous Region of China to the state key laboratory of reproductive regulation and breeding of grassland livestock(2019ZD031).

摘　　要：This study used alcoholic ammonium hydroxide to directly hydrolyze and extract secoisolariciresinol diglucoside(SDG)from flaxseed hull in a one pot reaction.The optimal extraction conditions,including the concentration of ammonium hydroxide,extraction time,and temperature,were examined in single factor experiments,followed by response surface methodology(RSM)with 3-level,3-factor Box-Behnken experiments.As a result,the optimal extraction conditions were determined as follows:material-liquid ratio 1:20,percentage of reagent ammonium hydroxide(25–28%of NH3 in water)in ethanol 33.7%(pH=12.9),extraction time 4.9 h,and extraction temperature 75.3°C.Under these conditions,the yield of SDG,as measured by ultra-high-performance liquid chromatography-mass spectrometry,was 23.3 mg/g,consistent with the predicted content of SDG in flaxseed hull(23.0 mg/g).Further,30.0 g of pulverized flaxseed hull was extracted under the optimal conditions,and the extract was subjected to a single run of macroporous resin chromatography to obtain 772.1 mg of a fraction with an SDG content exceeding 76.1%.Subsequent chromatography on Sephadex LH20,yielded 602.8 mg SDG of 98.0%purity,and the yield was 20.1 mg/g(2.0%)from flaxseed hulls.Thus,one-pot hydrolysis and extraction of SDG using alcoholic ammonium hydroxide is simple,and of high-yield.

关 键 词：Flaxseed hull SDG EXTRACTION Alkaline hydrolysis RSM 

分 类 号：O64[理学—物理化学]