糖尿病肾脏疾病小鼠肾组织环状RNA差异表达的研究  

Differential expression analysis of circular RNA in mice kidney with diabetic kidney disease

作　　者：胡芳[1] 韦晓虹 薛瑞 周丽媛 孙辽[1] HU Fang;WEI Xiaohong;XUE Rui(Department of Endocrinology,The Fifth Afiliated Hospital,Sun Yat-sen University,Zhuhai 519000,China)

机构地区：[1]中山大学附属第五医院内分泌科,珠海519000 [2]珠海市中西医结合医院心胸外科

出　　处：《中国糖尿病杂志》2023年第8期603-608,共6页Chinese Journal of Diabetes

基　　金：珠海市科技计划基金资助项目(ZH2202200088HJL)。

摘　　要：目的探讨STZ诱导的DKD小鼠环状RNA(circRNA)的差异表达。方法8只小鼠分为正常对照组(Con)、DKD组,每组各4只。采用circRNA芯片检测各组肾组织circRNA表达谱,筛选差异表达circRNA分子,采用实时荧光定量PCR验证差异表达circRNA分子在各组肾组织中的表达变化,采用TargetScan及miRanda软件预测差异表达circRNA可能作用的miRNA靶点。结果DKD组共筛选出177个差异表达circRNA(差异倍数≥1.2且P<0.05),其中62个circRNA上调,115个circRNA下调,表达上调前5的差异circRNA是mmu_circRNA_40538、mmu_circRNA_21098、mmu_circRNA_20027、mmu_circRNA_25333和mmu_circRNA_25074,表达下调前5的差异circRNA是mmu_circRNA_19015、mmu_circRNA_41646、mmu_circRNA_011174、mmu_circRNA_29639及mmu_circRNA_28869。与Con组比较,DKD组FBG、24 h UAlb、mmu_circRNA_25074表达升高(P<0.05或P<0.01),体重、mmu_circRNA_011174及mmu_circRNA_41646表达降低(P<0.05)。结论STZ诱导的DKD小鼠肾组织中circRNA呈差异表达,为验证circRNA在DKD中的作用提供依据。Objective To explore the expression of kidney circular RNA in mice with diabetic kidney disease(DKD)induced by STZ.Methods A total of 8 mice were averagely divided into control group and DKD group.Different expression circRNAs were detected and screened by circRNA microchip.Then they were confirmed by quantitative real-time PCR in kidney tissue and compared between control and DKD mice.Finally,miRNA that can bind to circRNA was predicted by TargetScan&miRandaR.IesultsA total of 177 different circRNA were found in DKD group(fold change≥1.2 and P<0.05).Among them,62 circRNAs were upregulated and 115 circRNAs were down-regulated.The top five upregulated circRNAs were mmu_circRNA_40538,mmu_circRNA_21098,mmu_circRNA_20027,mmu_circRNA_25333 and mmu_circRNA_25074.And the top five downregulated circRNAs were mmu_circRNA_19015,mmu_circRNA_41646,mmu_circRNA_011174,mmu_circRNA_29639 and mmu_circRNA_28869.Compared with Con group,FBG,24 hUAlb,and mmu._circRNA_2507 expression were increased(P<0.05 or P<0.01),while weight,mmu._circRNA_011174 and mmu_circRNA.41646 expression were decreased(P<0.05)in DKD group.Conclusion There were different expression circRNAs in kidney of STZ-induced DKD mice.It will provide the basis for further study on the possible involvement of circRNAs in the pathogenesis of DKD.

关 键 词：糖尿病肾脏疾病 环状RNA 基因芯片 靶基因 

分 类 号：R587.2[医药卫生—内分泌] R692.9[医药卫生—内科学]