微弧氧化TiO_(2)涂层微结构对细胞黏附性能的影响  

Effect of Microstructure of Micro-Arc Oxidation TiO_(2) Coating on Cell Adhesion Properties

作　　者：翦奉林 金凡亚[2] 冯军 李必文[1] 但敏[2] 杨涵 黄熠 唐国庆 JIAN Fenglin;JIN Fanya;FENG Jun;LI Biwen;DAN Min;YANG Han;HUANG Yi;TANG Guoqing(School of Mechanical Engineering,University of South China,Hengyang 421001,China;Southwest Institute of Nuclear Physics,Chengdu 610041,China)

机构地区：[1]南华大学机械工程学院,湖南衡阳421001 [2]核工业西南物理研究院,四川成都610041

出　　处：《材料保护》2023年第10期123-129,共7页Materials Protection

摘　　要：为改善TA2纯钛表面的生物相容性,采用微弧氧化技术,通过设置不同的电解液和电参数,在TA2纯钛基材上制备出4种具有不同微结构的氧化钛涂层,采用XRD、SEM、台阶仪、接触角测量仪测试样品的物相组成、微观形貌、粗糙度和亲水性,并以MC3T3-E1(小鼠成骨细胞)与各组具有不同微结构的氧化钛涂层样品共培养后,观察细胞与样品表面的黏附状态。结果表明:采用磷酸盐体系电解液可制备出具有大孔平整和大孔粗糙结构形貌的氧化钛涂层,采用SEM观察细胞的黏附状态发现,在具有这2种结构形貌的氧化钛涂层表面生成了丝状伪足,表面可见细胞分泌物,说明涂层表面具有一定的生物活性;采用NaOH体系电解液制备出具有细孔平整和细孔粗糙结构形貌的氧化钛涂层,在具有这2种结构形貌的氧化钛涂层表面,细胞的黏附面积明显增大,板状伪足和丝状伪足丰富,生物活性明显增强;进一步对比分析发现,氧化钛涂层在粗糙度较高时对细胞的迁移方向具有引导作用,在粗糙度较低时能够提升涂层表面对细胞的黏附能力,具有微纳二级结构的涂层对这2种能力具有整合作用。For improving the biocompatibility of TA2 pure titanium surface,micro-arc oxidation technology was used to prepare four kinds of titanium oxide coatings with different microstructures on TA2 pure titanium substrate by setting different electrolytes and electrical parameters.XRD,SEM,step profiler,and contact angle measuring instrument were employed to analyze the phase composition,micro morphology,roughness and hydrophilicity of coatings.Furthermore,MC3T3-E1(mouse osteoblasts)were co-cultured with the titanium oxide coatings with different microstructures to observe the adhesion status between cells and the surface of the sample.Results showed that titanium oxide coatings with structural morphology of smooth macropores and rough macropores could be prepared in phosphate system electrolyte.By observing the adhesion state of cells using SEM,it was found that filopodia were generated on the surface of titanium oxide coatings with these two structural morphology,and cell secretions could be seen on the surface,indicating that the coating surface had certain biological activity.Moreover,the titanium oxide coatings with structural morphology of smooth pores and small rough pores were prepared in NaOH system electrolyte.On the surface of the titanium oxide coating with these two structural morphology,the adhesion area of cells was significantly increased,lamellipodia and filopodia were abundant,and the biological activity was greatly enhanced.The further comparative analysis suggested that the titanium oxide coating could guide the migration direction of cells when the roughness was high,and could improve the adhesion ability of the coating surface to cells when the roughness was low.Besides,the coatings with micro and nano dual-order structure had an integration effect on the two kinds of abilities.

关 键 词：纯钛 氧化钛 微弧氧化 MC3T3-E1 生物活性 

分 类 号：TG174[金属学及工艺—金属表面处理]