Bottlenecks and recent advancements in detecting Mycobacterium tuberculosis in patients with HIV  

作　　者：Zixun Lin Liqin Sun Cheng Wang Fuxiang Wang Jun Wang Qian Li Hongzhou Lu 

机构地区：[1]National Clinical Research Centre for Infectious Diseases,The Third People's Hospital of Shenzhen,The Second Affiliated Hospital,Southern University of Science and Technology,Shenzhen,Guangdong,China [2]Department of Infectious Diseases,The Third People's Hospital of Shenzhen,The Second Affiliated Hospital,Southern University of Science and Technology,Shenzhen,Guangdong,China [3]School of Public Health,Bengbu Medical College,Bengbu,Anhui,China [4]Clinical Laboratory,The Fifth People's Hospital of Wuxi,Jiangnan University,Wuxi,Jiangsu,China

出　　处：《iLABMED》2023年第1期44-57,共14页智能检验医学（英文）

基　　金：the China Post‐Doctoral Science Foundation,Grant/Award Number:2022M711486;the Shenzhen Science and Technology Innovation Commission Project,Grant/Award Numbers:JCYJ20200109144203815,JCYJ20220530163406014;Basic and Applied Basic Research Fund of Guangdong Province:Regional Joint Fund‐Youth Fund Project,Grant/Award Number:2020A15151108031;The National Natural Science Foundation of China,Grant/Award Number:82072281;the Shenzhen Third People's Hospital project,Grant/Award Number:G2021027。

摘　　要：Tuberculosis(TB)remains a leading cause of deaths among patients with acquired immunodeficiency syndrome patients.Early diagnosis of TB is essential for administering timely anti‐TB therapy and improving health out-comes,particularly in the people living with HIV.However,conventional techniques used to detect Mycobacterium tuberculosis have significant draw-backs:for example,sputum smear microscopy has low sensitivity,and liquid culture is time‐consuming in patients with HIV‐TB co‐infection due to low sputum production.In addition,while immunological‐based methods involving tuberculin skin testing and interferon gamma release assays are commonly used for auxiliary TB diagnosis,they are often inaccurate in immunodeficient patients.Molecular techniques such as line probe assays,Xpert MTB,and lipoarabinomannan assay are recommended for early diagnosis by World Health Organization.However,no single technique is sufficicent for diagnosing HIV/TB co‐infection,suggesting that multiple diagnostic tests should be used to detect TB.Here,we summarize the drawbacks and advantages of existing TB‐diagnostic methods,as well as their applications to diagnosing HIV/TB co‐infection.We describe newly emerging technologies such as whole genome sequencing and mass spectrometry,with the aim of providing updated guidelines and alternative strategies for TB diagnosis,and particularly HIV/TB diagnosis.

关 键 词：diagnosis HIV/TB co‐infection TUBERCULOSIS 

分 类 号：R52[医药卫生—内科学]