网络药理学联合动物实验探讨润燥止痒胶囊治疗湿疹的作用机制  

Network pharmacology combined with animal experiment to explore the mechanism of action ofRunzao Zhiyang capsule in treating eczema

作　　者：胡辉莹 胡银霞 刘昌顺[2] 郝勇 Hu Huiying;Hu Yinxia;Liu Changshun;Hao Yong(General Hospital of Southern Theatre Command,People's Liberation Army of China,Guangzhou 510010,China;School of Traditional Chinese Medicine,Southern Medical University,Guangzhou 510515,China)

机构地区：[1]中国人民解放军南部战区总医院,广州510010 [2]南方医科大学中医药学院,广州510515

出　　处：《中国临床解剖学杂志》2023年第5期572-577,共6页Chinese Journal of Clinical Anatomy

基　　金：国家自然科学基金青年项目(82104729)。

摘　　要：目的 通过网络药理学和动物实验,揭示润燥止痒胶囊(RunZao ZhiYang capsule,RZZY治疗湿疹的药理学机制。方法 选取RZZY中23个成分为研究对象,利用数据库筛选方药靶点,使用Metascape数据库对靶点进行KEGG通路富集分析,Cytoscape软件构建“成分-靶点-通路”网络,筛选湿疹关联通路,开展动物实验评价RZZY对湿疹的疗效,验证RZZY对关键靶点调节作用。结果 RZZY与湿疹关联靶点72个,包括JAK2、STAT3、PIK3R1、AKT1、MAPK1等;KEGG富集得到AGE-RAGE、TNF、Th17 cell differentiation、PI3K-Akt等关键信号转导通路;动物研究发现RZZY显著减轻湿疹模型大鼠皮肤肿胀、表面溃烂和渗透,降低湿疹面积及严重度指数评分、皮肤厚度和病理评分,确证RZZY对湿疹关键靶点JAK2和STAT3蛋白有下调作用,与网络药理学预测结果相一致。结论 本研究联合网络药理学和动物实验验证RZZY对湿疹的作用机理,为其临床应用提供理论依据。Objective To reveal the pharmacological mechanism of RunZao ZhiYang capsule(RZZY)in treating eczema through network pharmacology and animal validation strategies.Methods Twenty-three components of RZZY were selected as research objects,and the target of prescription was screened by using the database,and the target was enriched by KEGG pathway by using Metascape database.The"composition-target-pathway"network was constructed by Cytoscape software,and the associated pathways of eczema were screened.Animal experiments were conducted to evaluate the efficacy of RZZY on eczema,and verify the regulatory effect of RZZY on key targets.Results There were 72 targets associated with RZZY and eczema,including JAK2,STAT3,PIK3R1,AKT1,MAPK1,etc.Key signal pathways such as AGE-RAGE,TNF,Th17 cell differentiation and PI3K-Akt have been enriched by KEGG.Animal studies revealed that RZZY significantly reduced skin swelling,surface ulceration and penetration in eczema model rats,and decreased eczema area and severity index score,skin thickness and pathological score,confirming the down-regulation effect of RZZY on key eczema targets JAK2 and STAT3 proteins,which was consistent with the predicted results of network pharmacology.Conclusions This study,combined with network pharmacology and in vivo animal experiments,reveals the mechanism of RZZY on eczema,providing theoretical basis for its clinical application.

关 键 词：润燥止痒胶囊 网络药理学 湿疹 动物验证 

分 类 号：R285[医药卫生—中药学]