细菌纳米纤维素/壳聚糖微球复合膜的制备及评价  

Preparation and Evaluation of Composite Membranes of Bacterial Nanocellulose/Chitosan Microspheres

作　　者：吴雯[1] 陈琳[1] 洪枫[1,2] WU Wen;CHEN Lin;HONG Feng(Research Group of Microbiological Engineering and Medical Materials,College of Biological Science and Medical Engineering,Donghua University,Shanghai 201620,China;National Advanced Functional Fiber Innovation Center,Suzhou 215000,China)

机构地区：[1]东华大学生物与医学工程学院微生物工程与医学材料研究组,上海201620 [2]国家先进功能纤维创新中心,江苏苏州215000

出　　处：《纤维素科学与技术》2023年第3期1-6,I0001,共7页Journal of Cellulose Science and Technology

基　　金：国家先进功能纤维创新中心重点项目(HX105210640);海南省重点研发计划项目(ZDYF2021GXJS025)。

摘　　要：以细菌纳米纤维素(BNC)膜为基底,在三聚磷酸钠(TPP)的存在下,通过离子凝胶法让壳聚糖(CS)分子在BNC网络内部形成微球(CSMS),获得BNC/CSMS复合膜,并对材料的理化性能和生物活性进行测试。结果表明:1.0%的TPP与1.0%或者1.5%的CS的浓度组合下,微球形态明显且分布均匀,粒径约40 nm;CS浓度增加提高了复合膜的力学和抗菌性能;0.5%和1.0%的CS制得材料更有助于细胞增殖;材料植入大鼠皮下后,无严重炎症反应,且促进细胞附着和细胞外基质重塑,证明其组织相容性良好,在伤口敷料上有很好的应用前景。BNC/CSMS composite membranes were obtained by using the ionic gelation method,where chitosan(CS)and sodium triphosphate(TPP)were used to make CS microspheres(CSMS)inside bacterial nanocellulose(BNC)nanofiber matrix.Physicochemical properties and biological activity of the materials including mechanical properties,antibacterial properties,cytocompatibility,and histocompatibility were performed.The results showed that the concentrations of CS and TPP affected the size and distribution of CSMS,and the combination of 1.0%TPP with 1.0%or 1.5%CS resulted in obvious morphology and uniform distribution of microspheres with a particle size of about 40 nm.All the composite membranes contained characteristic groups of CS.The increase of CS concentration improved the mechanical and antibacterial properties of the materials.The materials produced with 0.5%and 1.0%of CS were more supportive of cell proliferation.After the materials were implanted via subcutaneous embedding experiment in rats,the attachment of fibroblasts and the presence of abundant collagen fibers were seen,and there was a slight inflammation at the beginning,but then it disappeared,proving that the materials have excellent histocompatibility.All data and results indicate that BNC/CSMS has great potential in application as wound dressings.

关 键 词：细菌纳米纤维素 壳聚糖微球 离子凝胶法 伤口敷料 

分 类 号：R318.08[医药卫生—生物医学工程]