Establishment of enzyme-linked immunosorbent assay for beef and lamb contents in cooked meat  

作　　者：Yujing Li Jingjing Liu Sufang Fan Li Zhao Jing Zhang Erjing Zhang Ziran Li Yan Zhang Chunsheng Li 

机构地区：[1]Hebei Jiaotong Vocational and Technical College,Shijiazhuang 050035,China [2]Biology Institute of Hebei Academy of Sciences,Shijiazhuang 050081,China [3]Hebei Food Safety Key Labratory,Key Laboratory of Special Food Supenvision Technolog for State Market Regpulation,Hebei Engineering Research Center for Special Food Sagfery and Health,Hebei Food Inspection cand Research Instinute,Shijiazhuang 050227,China [4]College ofLife Sciences,Hebei Normal Universiny,Shijiazhuang 050024,China [5]Julu County Applied Technology Research Instinute,Jultu 055250,China

出　　处：《Journal of Future Foods》2024年第1期91-96,共6页未来食品学报（英文）

基　　金：This research was funded by Hebei Provincial Department of Science and Technology(21375501D);the Hebei Academy of Sciences(2019Q01).

摘　　要：In this study,an enzyme 1linked immunosorbent assay(ELISA)was established to detect beef and 1amb components,and its performance was tested.Double-antibody sandwich ELISA was adopted and determined a coating concentration of capture antibody 3G5 of 1:4000,a working concentration of enzyme-labeled antibody 2E7-horseradish peroxidase(HRP)of 1:1000,a sample incubation time of 60 min and a detection antibody reaction time of 60 min.The specificity,sensitivity,repeatability and stability of this assay were detemmined.The limit of detection for beef and 1amb skeleta1 muscle troponin I was 45 mg/kg,the inter-assay and intra-assay recovery rates ranged from 80.4%to 115.7%,the coefficients of variation were below 13.6%,and the cIoss reaction rates of the tissue components of chicken,duck and fish were below 13.4%.The sandwich ELISA method established in this study is stable and has high accuracy.The test results were consistent with the polymerase chain reaction(PCR)method at 50 and 100 g/kg-Therefore,this ELISA method can be used to quantitatively detect beef and 1amb components in meat products.

关 键 词：Double antibody sandwich enzyme-1inked immunosorbent assay(ELISA) Beef components Lamb components 

分 类 号：TS251[轻工技术与工程—农产品加工及贮藏工程]