两个鸭茅品种根系响应淹水胁迫的比较代谢组学分析  

Comparative metabolomics analysis of root systems of two Dactylis glomerata cultivars in response to submergence stress

作　　者：沈秉娜 尚盼盼 曾兵[1] 李林祥 杨兴云 毕磊 郑玉倩 屈明好 李文文 周晓丽[1] 饶骏[1] 曾兵 SHEN Bing-na;SHANG Pan-pan;ZENG Bing;LI Lin-xiang;YANG Xing-yun;BI Lei;ZHENG Yu-qian;QU Ming-hao;LI Wen-wen;ZHOU Xiao-li;RAO Jun;ZENG Bing(College of Animal Science and Technology,Southwest University,Chongqing 402460,China;Bazhong Academy of Agricultural and Forestry Sciences,Bazhong 636001,China;Chongqing University Herbivore Engineering Research Center,Chongqing 400715,China)

机构地区：[1]西南大学动物科学技术学院,重庆402460 [2]巴中市农林科学研究院,四川巴中636001 [3]重庆高校草食动物工程研究中心,重庆400715

出　　处：《草业学报》2023年第10期40-57,共18页Acta Prataculturae Sinica

基　　金：重庆市现代山地特色高效农业产业技术体系(草食牲畜)团队项目(2023);重庆高校草食动物工程研究中心建设项目;西南大学大学生创新训练项目(X202210635068)资助

摘　　要：鸭茅是世界著名温带冷季型牧草。近年来,我国部分地区涝害频发,严重影响饲草作物生产。为探究淹水胁迫下鸭茅根系的响应机制,以耐涝型鸭茅“滇北”(DB)和涝敏型鸭茅“安巴”(AB)为材料,设置2个淹水处理(时长8和24 h),以不淹水处理为对照(0 h),观察形态并对0、8和24 h 3个时间点的幼苗根系进行代谢组学分析。结果表明,淹水胁迫处理后,2个品种的鸭茅根系长势均变弱,根系变黑,且DB在处理24 h后产生了不定根。在DB_(8h)vs DB_(0h)组筛选出了120个差异代谢物;DB_(24h)vs DB_(0h)组筛选出155个差异代谢物;AB_(8h)vs AB_(0h)组筛选出93个差异代谢物;AB_(24h)vs AB_(0h)组筛选出118个差异代谢物;DB_(0h)vs AB_(0h)组筛选出了80个差异代谢物;DB_(8h)vs AB_(8h)组筛选出125个差异代谢物;DB_(24h)vs AB_(24h)组筛选出48个显著差异代谢物。在耐涝型鸭茅DB中发现了6个黄酮类化合物,即:橙皮素、甘草素、芹黄素、新橙皮苷、柚皮素和柚皮苷,参与的代谢途径是类黄酮生物合成途径;而在涝敏型鸭茅中发现4个氨基酸及其衍生物,分别是:O-磷酸-L-丝氨酸、L-谷氨酸、L-酪氨酸和色氨酸,参与的代谢途径是氨酰-tRNA生物合成、甘氨酸、丝氨酸和苏氨酸代谢、硫代葡萄糖苷生物合成、苯丙氨酸、酪氨酸和色氨酸生物合成以及色氨酸代谢途径。通过比对相同淹水胁迫时长下DB和AB的差异代谢物发现,DB中存在的差异代谢物主要是蔗糖、异麦芽糖、6-磷酸海藻糖、L-鸟氨酸、L-组氨酸、D-鸟氨酸和芹菜素,参与的代谢途径分别是ABC转运因子、淀粉和蔗糖代谢、β-丙氨酸代谢、类黄酮生物合成以及黄酮和黄酮醇的生物合成代谢途径。这些差异代谢物可能是2个品种耐涝性存在差异的原因。该结果为解析鸭茅响应淹水胁迫过程中的差异代谢物质和代谢途径奠定了基础,为进一步研究鸭茅对淹水胁迫调控的分子机理提供参Orchard grass(Dactylis glomerata)is a world famous temperate cold-season forage grass.In recent years,frequent floods in southern China have seriously affected forage crop production.In order to explore the response mechanism of orchard grass roots under submergence stress,the waterlogging-tolerant orchard grass cultivar‘Dianbei’(DB)and the waterlogging-sensitive orchard grass cultivar‘Anba’(AB)were studied.Two waterlogging treatments(8 and 24 h)were set up,and a non-waterlogging treatment was used as the control(0 h).The morphology was observed and metabolomics analysis was performed on the seedling roots for 0,8 and 24 h treatments.It was found that the root growth of the two varieties became weak and the roots became black after submergence stress treatment,and adventitious roots were produced by cultivar DB after treatment for 24 h.120 differentially produced metabolites were identified in the DB_(8h)vs DB_(0h)comparison and 155 differentially produced metabolites were identified in the DB_(24h)vs DB_(0h)comparison,while 93 differentially produced metabolites were identified in the AB_(8h)vs AB_(0h)comparison and 118 differentially produced metabolites were identified in the AB_(24h)vs AB_(0h)comparison.Between cultivars,80 differentially produced metabolites were identified in the DB_(0h)vs AB_(0h)comparison;125 differentially produced metabolites were identified in the DB_(8h)vs AB_(8h)comparison and 48 significantly differentially produced metabolites were identified in the DB24h vs AB_(24h)group.Six flavonoids involved in the metabolic pathway of flavonoid biosynthesis were found in the waterlogging-tolerant orchard grass cultivar DB,namely:Hesperidin,glycyrrhizin,apigenin,neohesperidin,naringin and naringenin;While four amino acids and their derivatives were found in the waterlogging-sensitive orchard grass cultivar,namely:O-phosphate-L-serine,L-glutamic acid,L-tyrosine and tryptophan.These amino acids are involved in the metabolic pathway of amyl-tRNA biosynthesis;Glycine,serine and threonine me

关 键 词：鸭茅 淹水胁迫 代谢组学 差异代谢物 代谢通路 

分 类 号：S543.3[农业科学—作物学]