大狼毒对紫花苜蓿幼苗生理及内源激素含量的影响  

Effects of Euphorbia jolkinii on physiology and endogenous hormone content of alfalfa seedlings

作　　者：单贵莲[1] 马祖艳 李嘉懿 刘洋 谢勇 刘佳 初晓辉[1] SHAN Gui-lian;MA Zu-yan;LI Jia-yi;LIU Yang;XIE Yong;LIU Jia;CHU Xiao-hui(Department of Grassland Science,Yunnan Agricultural University,Kunming 650201,China;Dezhou Qingyun County Natural Resources Bureau,Dezhou 253700,China;Forestry and Grassland Bureau of Yunnan Province,Kunming 650224,China;Kunming Municipal Animal Disease Prevention and Control Center,Kunming 650223,China;Shangri La Forestry and Grassland Bureau,Diqing 674499,China)

机构地区：[1]云南农业大学草业科学系,云南昆明650201 [2]德州市庆云县自然资源局,山东德州253700 [3]云南省林业和草原局,云南昆明650224 [4]云南省昆明市动物疫病预防控制中心,云南昆明650223 [5]香格里拉市林业和草原局,云南迪庆674499

出　　处：《草业学报》2023年第10期153-161,共9页Acta Prataculturae Sinica

基　　金：云南省农业联合专项(202101BD070001-068);云南省万人计划青年拔尖人才专项项目资助。

摘　　要：为探明大狼毒所含化感物质对牧草产生的影响,促进毒害草型退化草地的补播治理,本研究采用室内控制实验,开展大狼毒对苜蓿幼苗形态、生理及内源激素含量的影响研究,结果表明:大狼毒植株粉碎物的添加导致紫花苜蓿根系活力下降,叶绿素及赤霉素(GA)、玉米素(ZT)、吲哚乙酸(IAA)合成受阻,致使紫花苜蓿幼苗生长受到抑制。根部土壤粉碎物的添加一方面促进了紫花苜蓿叶绿素的合成,提高其根系活力,另一方面又阻碍了GA、ZT、IAA的合成,最终表现为对紫花苜蓿幼苗生长指标(根长和单株鲜重)无显著影响。综上,大狼毒植株含有的化感物质会对紫花苜蓿幼苗激素合成产生强烈的抑制作用,由此影响紫花苜蓿幼苗的生长。This research explored the effects of allelochemicals in Euphorbia jolkinii on alfalfa(Medicago sativa)seedlings using an indoor controlled experiment with the aim of providing information to support the replanting of improved forage species and restoration of degraded grassland where the poisonous herb has spread.We determined the effects of E.jolkinii on the morphology,physiology and endogenous hormone content of alfalfa seedlings.It was found that the addition of E.jolkinii reduced seedling root activity initially,and then blocked the synthesis of chlorophyll,gibberellic acid(GA),zeatin(ZT)and indoleacetic acid(IAA),with the ultimate effect of significant inhibition of alfalfa seedling growth.The addition of soil from the root zone of E.jolkinii plants promoted the synthesis of chlorophyll and improved the root activity of alfalfa on the one hand,and reduced the synthesis of GA,ZT,and IAA on the other hand,with the overall result was that there was no significant effect on the growth index of alfalfa seedlings in terms of seedling root length and fresh weight in treatments where root-zone soil was added.In summary,the allelochemicals contained in E.jolkinii can strongly inhibit the hormone synthesis of alfalfa seedlings,and thereby inhibit the growth of alfalfa seedlings but some amelioration through inoculation with E.jolkinii root-zone soil is possible.

关 键 词：大狼毒 紫花苜蓿 幼苗形态 抗氧化酶 内源激素 化感效应 

分 类 号：S812.6[农业科学—草业科学] S541.9[农业科学—畜牧学]