超表达CsCslE1_4基因对烟草细胞壁成分的影响  

Effects of Overexpression of CsCslE1_4 Gene on Cell Wall Composition of Tobacco

作　　者：姚新转 李倩倩 吕立堂 YAO Xinzhuan;LI Qianqian;L Litang(College of Tea,Guizhou University,Guiyang 550025,China;Key Laboratory of Mountain Plant Resources Protection and Germplasm Conservation Innovation of Ministry of Education,Institute of Agricultural Bioengineering,College of Life Sciences,Guizhou University,Guiyang 550025,China)

机构地区：[1]贵州大学茶学院,贵阳550025 [2]贵州大学生命科学学院/农业生物工程研究院/山地植物资源保护与种质创新省部共建教育部重点实验室,贵阳550025

出　　处：《种子》2023年第8期11-15,20,共6页Seed

基　　金：贵州省科技计划项目(黔科合支撑[2021]一般111);国家自然科学基金“CsHSF24.1调控茶树儿茶素合成的分子机制解析”(32260789);贵州省优秀青年科技人才培养项目(黔科合平台人才[2019]5651)。

摘　　要：类纤维素合酶E(CslE)基因与纤维素合成相关,决定着细胞形状以及大小,并在植物生长发育阶段起着机械支撑和抵抗病原物侵染的作用。为研究CsCslE1_4基因对植物细胞壁的重要组成成分的影响,应用转基因烟草研究CsCslE1_4基因的基本功能,通过农杆菌介导法遗传转化烟草,经鉴定得到18株阳性植株,结合定量表达以及细胞壁组成成分测定。结果表明,与野生型相比,转基因株系茎的纤维素含量是野生型的1.43~5.48倍,叶中纤维素含量除株系CslE2外,其余株系纤维素含量是野生型的1.08~2.49倍,大部分转基因株系半纤维素含量是野生型半纤维素含量的1.18~2.22倍,但果胶含量变化趋势与纤维素和半纤维素含量变化趋势相反,木质素含量没有明显的变化。CsCslE1_4基因可以促进纤维素合成,这为后续解析基因功能以及抗逆性提供依据。Cellulose synthase,similar to the E(CslE)gene,is associated with cellulose synthesis,determining cell shape and size and plays a role in mechanical support and resistance to pathogen infection during plant growth and development.In order to study CsCslE1_4 genes influence on important components of plant cell walls,transgenic tobacco was used to study CsCslE1_4 genes basic functions.Through Agrobacterium mediated genetic transformation of tobacco,18 positive plants were identified.Combined with quantitative expression and determination of cell wall composition,the results showed that compared with the wild type,the cellulose content of the transgenic lines was 1.43 to 5.48 times that of the wild type,the cellulose content of the leaves was 1.08 to 2.49 times that of the wild type except CslE2,and the hemicellulose content of most transgenic lines was 1.18 to 2.22 times that of the wild type hemicellulose,However,the change trend of pectin content was opposite to that of cellulose and hemicellulose content,and lignin content did not change significantly.CsCslE1_4 genes could promote cellulose synthesis,providing a theoretical basis for subsequent analysis of gene function and stress resistance.

关 键 词：烟草 纤维素合酶 细胞壁 遗传转化 纤维素 

分 类 号：S572[农业科学—烟草工业]