半夏组织培养一步成苗技术初步研究  被引量:1

Preliminary Study on the Technology of One-step Culture in Pinellia ternate

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作  者:程搏幸 罗夫来[2] 李冀青 王洪亮 李开阳 周丹 CHENG Boxing;LUO Fulai;LI Jiqing;WANG Hongliang;LI Kaiyang;ZHOU Dan(School of Biological Sciences,Guizhou Education University,Guiyang 550018,China;College of Agriculture,Guizhou University,Guiyang 550025,China;Guizhou Agricultural Science and Technology Development Center,Guiyang 550001,China;Hezhang County Mountain High Efficiency Agricultural Science and Technology Limited Company,Bijie Guizhou 553200,China)

机构地区:[1]贵州师范学院生物科学学院,贵阳550018 [2]贵州大学农学院,贵阳550025 [3]贵州省农业科技发展中心,贵阳550001 [4]赫章县山地高效农业科技有限公司,贵州毕节553200

出  处:《种子》2023年第8期147-151,共5页Seed

基  金:贵州省科技计划项目(黔科合支撑[2020]4Y113号、黔科合支撑[2020]4Y108号、黔科合支撑[2021]一般245);贵州省普通高等学校青年科技人才成长项目(黔教合KY字[2021]233);贵州师范学院2020年度校级博士课题(2020BS004)。

摘  要:为简化半夏组织培养种苗生产流程,缩短培育周期,建立半夏一步成苗技术,以半夏为试验材料,采用二因素三水平完全随机设计筛选最适外植体及NAA、6-BA浓度配比,并探讨炼苗时间、移栽基质、移栽基质含水量对幼苗移栽成活率及长势的影响。结果表明,以叶片作为外植体,用MS基本培养基+1.5 mg/L NAA+1 mg/L 6-BA+0.1%活性炭作为培养基有利于半夏一步成苗;组培苗移栽炼苗的最适条件是:闭瓶炼苗2 d、开瓶炼苗3 d,以腐殖土+蛭石为移栽炼苗基质(含水量80%)。In order to simplify the tissue culture procedures for Pinellia ternate,shorten the incubation period,and develop a one-step seedling technology of Pinellia ternate preliminarily.The optimal explants for Pinellia ternate were selected,and the appropriate concentration ratio of NAA and 6-BA were determined with factors at two and three levels.The effects of acclimatization time,substrate for transplantation,and substrate water content on survival rates and growth of transplanted seedlings were also investigated.Result showed that using leaves as explants and MS medium plus 0.1%activated charcoal,1.5 mg/L NAA and 1 mg/L 6-BA were conducive to one-step seedling formation of Pinellia ternate.Most appropriate conditions for acclimatization of tissue-cultured seedlings were as follows:using humus and vermiculite as substrate for transplantation(water content 80%)and hardening seedlings in closed-cap bottles for 2 days and opened-cap bottles for 3 days.

关 键 词:半夏 组织培养 一步成苗 移栽炼苗 

分 类 号:S567.239[农业科学—中草药栽培]

 

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