止眩方气雾给药预防晕动症用于小鼠的效果及基于网络药理学的机制预测  

Effect and Mechanism Prediction of Zhixuan Prescription in Preventing Motion Sickness in Mice by Aerosol Administration Based on Network Pharmacology

作　　者：邓竺 郑佳欣 张瑞颖 杨钦翔 徐佳怡 袁省之 刘丹彤 DENG Zhu;ZHENG Jiaxin;ZHANG Ruiying;YANG Qinxiang;XU Jiayi;YUAN Xingzhi;LIU Dantong(The First Clinical Medical College,Beijing University of Chinese Medicine,Beijing 100700,China;School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 102488,China;School of Traditional Chinese Medicine,Beijing University of Chinese Medicine,Beijing 100029,China)

机构地区：[1]北京中医药大学第一临床医学院,北京100700 [2]北京中医药大学中药学院,北京102488 [3]北京中医药大学中医学院,北京100029

出　　处：《中国中医药现代远程教育》2023年第21期155-159,共5页Chinese Medicine Modern Distance Education of China

基　　金：北京市大学生创新创业训练计划项目【No.S202110026002】。

摘　　要：目的观察止眩方气雾给药预防晕动症的疗效,并通过网络药理学预测其作用机制。方法提前给药预防晕动症昆明小鼠模型,利用行为学实验和晕反应指数探究止眩方的疗效。利用中药系统药理学数据库与分析平台(TCMSP)和文献挖掘获取止眩方有效成分和靶点,运用Cytoscape 3.7.1软件构建“药对-化合物-通路-疾病”网络和蛋白相互作用(PPI)网络。在Metascape数据库中对靶点进行京都基因和基因组百科全书(KEGG)分析。结果旷场实验、高架十字迷宫实验结果均显示雾化组小鼠活跃度高于模型组,且雾化高浓度组的晕反应指数相比于模型组明显降低(P<0.05)。止眩方有130个靶点与晕动症相互作用。关键靶点作用于丝裂原活化蛋白激酶(MAPK)、磷脂酰肌醇3-激酶(PI3K)-丝氨酸/苏氨酸激酶(Akt)等193条信号通路。结论雾化给予止眩方能够预防小鼠晕动症,疗效明确,且止眩方预防晕动症具有多成分、多靶点、多通路的作用特点,为后续研究奠定基础。Objective To observe the effect of Zhixuan prescription aerosol administration on preventing motion sickness and predict-ing its mechanism by network pharmacology.Methods After drug-induced motion sickness model was established in KM mice,and the formula’s efficacy was investigated with behavioristics tests and motion sickness index.The formula’s active ingredients and tar-gets were obtained in TCMSP and by literature mining.The KEGG pathways of the targets were analyzed in Metascape database.Re-sults The open field and elevated cross-maze results all showed that compared with the model group,the mice in the aerosol group were more active,and the motion sickness index was significantly lower in the aerosol high-concentration group(P<0.05).There were 130 targets related to motion sickness in Zhixuan Fang.The key targets acted on 193 signaling pathways,including MAPK and PI3K-Akt signaling pathways.Conclusion The aerosol administration of Zhixuan prescription can prevent motion sickness in mice,and it has multi-component,multi-target,multi-channel characteristics,which lay the foundation for subsequent research.

关 键 词：注车 晕动症 中药 雾化吸入 行为学 网络药理学 实验研究 

分 类 号：R285.5[医药卫生—中药学]