一株望天树根际高效解磷细菌解磷能力的研究  

Study on phosphate-solubilizing ability of a high efficiency phosphate-solubilizing bacterium in rhizosphere of Parashorea chinensis Wang Hsie

作　　者：田湘 韩小美 黄平升 李万年 杨钦潮 杨梅[2] TIAN Xiang;HAN Xiao-mei;HUANG Ping-sheng;LI Wan-nian;YANG Qin-chao;YANG Mei(Nanning Liangfengjiang National Forest Park,Nanning 530004,China;College of Forestry,Guangxi University,Nanning 530004,China)

机构地区：[1]广西南宁良凤江国家森林公园,南宁530004 [2]广西大学林学院,南宁530004

出　　处：《湖北农业科学》2023年第9期51-56,共6页Hubei Agricultural Sciences

基　　金：国家自然科学基金项目(31960307);广西自然科学基金项目(2018GXNSFAA28110);广西自筹经费林业科技项目(桂林科研[2022ZC]第77号)。

摘　　要：以1株来源于广西南宁树木园望天树(Parashorea chinensis Wang Hsie.)根际土壤的高效解无机磷细菌P4[洋葱伯克霍尔德菌(Burkholderia cepacia)]为材料,研究了解磷细菌P4在培养168 h内解磷能力和菌液pH的动态变化情况,比较了菌株在不同温度、pH、碳源、氮源、碳氮比(C/N)和NaCl浓度条件下的解磷能力,以期为解磷微生物在微生物菌肥生产上的开发和应用提供理论依据。结果表明,在培养168 h内,菌株P4培养液中有效磷含量呈先升高后降低的趋势,pH则呈先降低后升高的趋势,在培养120 h时菌液中有效磷含量最高,为552.87 mg/L,此时菌液pH最低;相关性分析表明,菌株P4溶磷量与培养液pH间存在极显著的负相关;菌株P4在培养温度为30℃、pH为6.5、碳源为乳糖、氮源为草酸铵、C/N为20∶1、NaCl浓度为0.5%时解无机磷效果最好且生长状况良好,在后续微生物菌肥研制中具有较大潜力。A highly efficient inorganic phosphorus degrading bacterium P4(Burkholderia cepacia)from the rhizosphere soil of the Parashorea chinensis Wang Hsie.in Nanning Arboretum in Guangxi was used as the research material,the dynamic changes of phosphate-solubilizing ability and pH of phosphate-solubilizing bacteria P4 within 168 h were studied,and the phosphate-solubilizing ability of phosphorus bacteria P4 under different temperature,pH,carbon source,nitrogen source,C/N and NaCl concentration was compared,in order to provide a theoretical basis for the development and application of phosphate-solubilizing microorganisms in microbial fertilizer production.The results showed that,within 168 h of culture,the content of available phosphorus in the culture medium of strain P4 increased first and then decreased,while the pH of the culture medium decreased first and then increased.At 120 h of culture,the content of available phosphorus in the culture medium was the highest,which was 552.87 mg/L,and the pH of the culture medium was the lowest.Correlation analysis showed that there was a highly significant negative correlation between the amount of phosphorus dissolved by strain P4 and the pH of the culture medium.The strain P4 had the best effect of hydrolyzing inorganic phosphorus and grew well when the temperature was 30℃,pH was 6.5,carbon source was lactose,nitrogen source was ammonium oxalate,C/N was 20∶1 and NaCl concentration was 0.5%.It had great potential in the follow-up development of microbial fertilizer.

关 键 词：解磷细菌 解磷能力 根际 望天树(Parashorea chinensis Wang Hsie.) 

分 类 号：S154.39[农业科学—土壤学]