莽草酸对PEDV感染仔猪生长性能、腹泻指数、血清免疫及抗氧化指标的影响  

Effect of shikimic acid on growth performance,diarrhea index,serum immunity and antioxidant indicators in piglets infected with PEDV

作　　者：赵雯 何颖 韦明宇 蒋家霞 张胜斌 胡庭俊[2] 张宁 赵凌[1] ZHAO Wen;HE Ying;WEI Ming-yu;JIANG Jia-xia;ZHANG Sheng-bin;HU Ting-jun;ZHANG Ning;ZHAO Ling

机构地区：[1]华中农业大学农业微生物学国家重点实验室,湖北武汉430070 [2]广西大学,广西南宁530004 [3]广西壮族自治区兽医研究所、广西兽医生物技术重点实验室、农业农村部、中国(广西)-东盟跨境动物疫病防控重点实验室,广西南宁530001 [4]广西农垦永新畜牧集团有限公司,广西南宁530022 [5]广西职业技术学院,广西南宁530226 [6]广西农垦永新畜牧集团西江公司,广西贵港537100

出　　处：《饲料研究》2023年第18期12-16,共5页Feed Research

基　　金：南宁市科技开发计划(项目编号:20222043);贵港市科技开发计划(项目编号:贵科技2203011);玉林市科学技开发计划(项目编号:玉市科20220515);广西兽医生物技术重点实验室开放基金(项目编号:22-035-32-B-01/02);广西兽医生物技术重点实验室开放基金(项目编号:23-035-32-B-02);广西基本科研业务费专项(项目编号:桂科专项23-3);广西农业科技自筹经费项目(项目编号:Z202223)。

摘　　要：试验旨在研究莽草酸对感染猪流行性腹泻病毒(PEDV)仔猪的生长性能、腹泻指数、血清免疫及抗氧化指标的影响。将120头感染PEDV的7日龄“杜×长×大”仔猪随机分为6组,每组5个重复,每个重复4头猪。处理1组、2组、3组分别每日给感染PEDV仔猪灌服800、400、200 mg/kg莽草酸,处理4组每日给感染PEDV仔猪灌服400 mg/kg绿原酸,处理5组每日给感染PEDV仔猪灌服2万单位庆大霉素,处理6组为阳性对照组,另选选择20头健康仔猪作为空白对照组。试验期为5 d,之后观察3 d。结果显示,与阳性对照组相比,处理1组、2组、3组仔猪平均日增重(ADG)均显著升高(P<0.05)。处理1组和2组仔猪的PEDV感染治愈率均达到85%,处理5组仔猪的PEDV感染治愈率为65%;处理1组、2组、3组仔猪血清中的免疫球蛋白G(IgG)含量均显著升高(P<0.05);处理1组、2组、3组仔猪血清中的总超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、一氧化氮(NO)、髓过氧化物酶(MPO)、谷胱甘肽过氧化物酶(GSH-Px)活性及总抗氧化能力(T-AOC)显著升高(P<0.05);丙二醛(MDA)、黄嘌呤氧化酶(XOD)水平显著降低(P<0.05)。研究表明,莽草酸能够提高仔猪的生长性能,降低腹泻指数,增强血清免疫力和抗氧化能力,对PEDV感染仔猪具有抗病促生长作用。The experiment was conducted to investigate the effect of shikimic acid on growth performance,diarrhea index,serum immunity and antioxidant indicators of piglets infected with porcine epidemic diarrhea virus(PEDV).A total of 120 clinically confirmed PEDV infected seven-day-old piglets(Duroc×Landrace×Yorkshire)were randomly divided into six groups,with five replicates in each group and four pigs in each replicate.Treatment group 1,group 2 and group 3 were administered 800,400 and 200 mg/kg of shikimic acid daily to piglets infected with PEDV,treatment group 4 were treated with daily administration of 400 mg/kg chlorogenic acid to piglets infected with PEDV,treatment group 5 were treated with 20000 units of gentamicin per day to piglets infected with PEDV,treatment group 6 were treated as positive control groups,and another 20 healthy piglets were selected as the blank control group.The experiment lasted for 5 d,and then observed for 3 d.The results showed that compared with control group with positive PEDV infection,the ADG of piglets with treatment group 1,group 2 and group 3 was significantly increased(P<0.05).The cure rate of PEDV infection in treatment group 1 and group 2 reached 85%,and the cure rate in treatment group 5 was 65%.The IgG content in the serum of piglets in group 1,group 2 and group 3 was significantly increased(P<0.05).The activity of SOD,CAT,NO,MPO,GSH-Px and T-AOC in the serum of piglets in group 1,group 2 and group 3 were significantly increased(P<0.05),and the content of MDA and XOD was significantly reduced(P<0.05).The experiment indicates that shikimic acid can improve the growth performance of piglets,reduce diarrhea index,enhance immunity and serum antioxidant capacity,and have a disease resistance and growth promoting effect on PEDV infected piglets.

关 键 词：莽草酸 猪流行性腹泻病毒(PEDV) 生长性能 抗氧化功能 

分 类 号：S828[农业科学—畜牧学]