UPLC-Q-TOF-MS^(E)技术研究白头翁皂苷在正常和溃疡性结肠炎模型大鼠体内代谢  

Study on metabolism of Pulsatilla saponin in normal and ulcerative colitis model rats by UPLC-Q-TOF-MS^(E)technology

作　　者：陈龙 张民权 司梦洁 欧阳梦琪 周良良 陈振华 CHEN Long;ZHANG Min-quan;SI Meng-jie;OUYANG Meng-qi;ZHOU Liang-liang;CHEN Zhen-hua(School of Pharmacy,Jiangxi Science and Technology Normal University,Jiangxi Province Key Laboratory of Drug Design and Evaluation,Nanchang 330013,China)

机构地区：[1]江西科技师范大学药学院,江西省药物分子设计与评价重点实验室,江西南昌330013

出　　处：《药学学报》2023年第9期2754-2762,共9页Acta Pharmaceutica Sinica

基　　金：江西省杰出青年基金(20224ACB216019);江西省自然科学基金青年基金(20202BABL216026);江西省药物分子设计与评价重点实验室开放基金(JKLDE-EF-2104);江西科技师范大学大学生创新创业项目(20221604120)。

摘　　要：本研究采用超高效液相色谱-四极杆-飞行时间质谱-全信息串联质谱(UPLC-Q-TOF-MS^(E))联用技术结合UNIFI分析平台快速分析鉴定常春藤皂苷元3-O-α-L-吡喃鼠李糖基-(1→2)-[β-D-吡喃葡萄糖基-(1→4)]-α-L-吡喃阿拉伯糖苷(白头翁皂苷D)、齐墩果酸3-O-α-L-吡喃鼠李糖基-(1→2)-[β-D-吡喃葡萄糖基-(1→4)]-α-L-吡喃阿拉伯糖苷(白头翁皂苷B_(7))和常春藤皂苷元3-O-α-L-吡喃鼠李糖基-(1→2)-α-L-吡喃阿拉伯糖苷(白头翁皂苷BD)在正常和溃疡性结肠炎(ulcerative colitis,UC)模型大鼠血浆及结肠组织中的代谢产物。主要依据化合物精确分子量、保留时间、中性丢失以及文献报道数据等信息建立数据库和分析方法,然后通过与空白对照组比较,结合偏差和化合物裂解规律筛选获得最终数据。结果表明,白头翁皂苷D、B_(7)和BD在正常和UC模型大鼠血浆及结肠组织中主要发生脱糖、羟基化和脱羟基化、甲基化和脱甲基化、脱氢、脱羧、水解反应。通过本文研究,将阐明白头翁皂苷D、B_(7)和BD在大鼠体内的代谢转化,确定进入体内的原形成分及其代谢物,以及结肠损伤是否会对它们的体内代谢产生影响,以探索白头翁皂苷D、B_(7)和BD原形或代谢产物中可能的抗结肠炎有效成分。本实验获得江西科技师范大学动物伦理委员会批准(批准号:Y202227)。In this study,ultra performance liquid chromatography-quadrupole-time of flight mass spectrometerMS^(E)(UPLC-Q-TOF-MS^(E))combined with UNIFI analysis platform was used to rapidly analyze and identify the metabolites of hederagenins 3-O-α-L-rhamnosyl-(1→2)-[β-D-glucopyranosyl-(1→4)]-α-L-arabopyranoside(Pulsatilla saponin D)and oleanolic acid 3-O-α-L-rhamnosyl-(1→2)-[β-D-glucopyranosyl-(1→4)]-α-L-arabopyran‐oside(Pulsatilla saponin B7)and hederagenins 3-O-α-L-rhamnosyl-(1→2)-α-L-arabopyranoside(Pulsatilla saponin BD)in plasma and colonic tissue of normal and ulcerative colitis(UC)rats.The database and analysis methods were established based on the precise molecular weight of compounds,retention time,neutral loss and reported data,and then the final data were obtained by comparing with the blank control group,combining with the deviation and the cracking rule of the compound.The results showed that the glucoses,hydroxylation and dehydroxyl‐ation,methylation and demethylation,dehydrogenation,decarboxylation and hydrolysis of saponin D,B7 and BD occurred in the plasma and colon tissues of normal and UC model rats.This study will clarify the metabolic trans‐formation of Pulsatilla saponins D,B7 and BD in rats,determine the prototype components and their metabolites that enter the body,and whether colon injury will affect their metabolism in vivo,so as to explore the possible anticolitis effective components in the prototype or metabolites of Pulsatilla saponins D,B7 and BD.This experiment was approved by Animal Ethics Committee of Jiangxi Science and Technology Normal University(approval number:Y202227).

关 键 词：UPLC-Q-TOF-MS^(E) 白头翁皂苷 代谢产物 溃疡性结肠炎 

分 类 号：R917[医药卫生—药物分析学]