Mas1受体在人胎盘中的表达及在子痫前期患者胎盘滋养层细胞中的生物学功能  

Expression of Mas1 receptor in human placenta and its effect on the function of trophoblast cells in pre-eclampsia patients

作　　者：崔恬玉 刘瑞霞 陈永杰 阴赪宏[1] Cui Tianyu;Liu Ruixia;Chen Yongjie;Yin Chenghong(Department of Central Laboratory,Beijing Obstetrics and Gynecology Hospital,Capital Medical University,Beijing 100026,China)

机构地区：[1]首都医科大学附属北京妇产医院中心实验室,北京100026

出　　处：《中华预防医学杂志》2023年第9期1426-1433,共8页Chinese Journal of Preventive Medicine

基　　金：首都医科大学附属北京妇产医院中青年学科骨干培养专项(FCYY201723);国家重点研发计划(2016YFC1000100)。

摘　　要：目的研究不同妊娠时期Mas1受体在健康孕妇胎盘中的表达变化,分析Mas1受体在子痫前期(PE)患者胎盘中的表达水平,及在滋养层细胞中的生物学功能。方法收集孕早期、孕中期、孕晚期正常孕妇胎盘绒毛组织,并从孕早期绒毛组织中分离培养人滋养层干细胞,通过免疫荧光化学和实时荧光定量PCR检测不同妊娠时期胎盘中Mas1受体表达情况;采用病例对照研究方法,选择足孕PE患者作为病例组,同期健康足孕产妇为对照组,收集两组产妇胎盘绒毛组织,利用免疫荧光化学和免疫蛋白印迹,研究PE时Mas1受体表达变化;采用HTR8/Svneo和BeWo细胞,Mas1受体激动剂和阻断剂干预,通过CCK8增殖实验和划痕实验检测Mas1受体对滋养层细胞增殖和迁移功能的影响。结果孕早期纳入8例,孕中期纳入7例,孕晚期纳入6例,正常胎盘绒毛组织中Mas1受体主要表达于人滋养层干细胞膜和胞质,且绒毛组织中Mas1受体m RNA孕晚期表达量明显高于孕中期;病例组纳入24例,对照组纳入12例。与对照组相比病例组胎盘绒毛中Mas1受体表达显著降低;激活/抑制Mas1受体对HTR8/Svneo细胞和BeWo细胞增殖活性无影响;激活Mas1受体对HTR8/Svneo细胞迁移能力无影响。结论Mas1受体在胎盘绒毛组织中存在表达且表达量随孕期发生变化,子痫前期患者Mas1受体表达减少,但其不影响滋养层细胞的增值和迁移功能。ObjectiveTo investigate the expression change of the Mas1 receptor in the placenta of healthy pregnant women during different gestation periods,analyze the expression level of the Mas1 receptor in the placenta of pre-eclampsia(PE)patients,and its biological function in trophoblast cells.MethodsPlacental villous tissues were collected from normal pregnant women in early,mid and late pregnancy.Human trophoblast stem cells were isolated and cultured from early pregnancy villous tissues.The expression of the Mas1 receptor was detected by fluorescence immunoassay and real-time fluorescence quantitative PCR.In a case-control study,patients with full-term PE were selected as the case group and healthy women with full-term pregnancy were selected as the control group.Placental villus tissues were collected from both groups.Immunofluorescence chemistry and immunoprotein blotting were used to study the changes in Mas1 receptor expression in PE.Mas1 receptor agonists and blockers induced HTR8/Svneo cells and BeWo cells,and the effects of the Mas1 receptor on the proliferation and migration of trophoblast cells were detected by the CCK8 proliferation test and scratch test.ResultsEight cases were included in early pregnancy,seven cases in mid-pregnancy and six cases in late pregnancy.Mas1 receptors in normal placental villi tissue were mainly expressed in human trophoblast stem cell membranes and cytoplasm,and the expression of Mas1 receptor mRNA in villi tissue was significantly higher in late pregnancy than in mid-pregnancy.There were 24 cases included in the case group and 12 cases in the control group.Mas1 receptor expression in placental villi was significantly lower in the case group compared to the control group;Activation/inhibition of the Mas1 receptor had no significant effect on the proliferation of HTR8/Svneo cells and BeWo cells.Activated Mas1 receptor had no significant effect on the migration ability of HTR8/Svneo cells.ConclusionMas1 receptors are expressed in placental villous tissue and their expression vari

关 键 词：Mas1受体 子痫前期 滋养层细胞 病例对照 

分 类 号：R714.244[医药卫生—妇产科学]