Inhibiting WNT secretion reduces high bone mass caused by Sost loss-of-function or gain-of-function mutations in Lrp5  

作　　者：Cassandra R.Diegel Ina Kramer Charles Moes Gabrielle E.Foxa Mitchell J.McDonald Zachary B.Madaj Sabine Guth Jun Liu Jennifer L.Harris Michaela Kneissel Bart O.Williams 

机构地区：[1]Department of Cell Biology,Van Andel Institute,333 Bostwick Ave.,NE,Grand Rapids,MI 49503,USA [2]Diseases of Aging and Regenerative Medicine,Novartis Institutes for Biomedical Research,CH-4002 Basel,Switzerland [3]Bioinformatics and Biostatistics Core,Van Andel Institute,333 Bostwick Ave.,NE,Grand Rapids,MI 49503,USA [4]Oncology,Novartis Institutes for Biomedical Research,San Diego,CA 92121,USA

出　　处：《Bone Research》2023年第3期595-606,共12页骨研究（英文版）

摘　　要：Proper regulation of Wnt signaling is critical for normal bone development and homeostasis.Mutations in several Wnt signaling components,which increase the activity of the pathway in the skeleton,cause high bone mass in human subjects and mouse models.Increased bone mass is often accompanied by severe headaches from increased intracranial pressure,which can lead to fatality and loss of vision or hearing due to the entrapment of cranial nerves.In addition,progressive forehead bossing and mandibular overgrowth occur in almost all subjects.Treatments that would provide symptomatic relief in these subjects are limited.Porcupine-mediated palmitoylation is necessary for Wnt secretion and binding to the frizzled receptor.Chemical inhibition of porcupine is a highly selective method of Wnt signaling inhibition.We treated three different mouse models of high bone mass caused by aberrant Wnt signaling,including homozygosity for loss-of-function in Sost,which models sclerosteosis,and two strains of mice carrying different point mutations in Lrp5(equivalent to human G171V and A214V),at 3 months of age with porcupine inhibitors for 5–6 weeks.Treatment significantly reduced both trabecular and cortical bone mass in all three models.This demonstrates that porcupine inhibition is potentially therapeutic for symptomatic relief in subjects who suffer from these disorders and further establishes that the continued production of Wnts is necessary for sustaining high bone mass in these models.

关 键 词：HOMEOSTASIS function mass 

分 类 号：R68[医药卫生—骨科学] R-332[医药卫生—外科学]