HPLC-ELSD测定灵芝孢子粉及产品中游离脂肪酸的含量  

Determination of free fatty acids in Ganoderma lingzhi spore powder and related products by HPLC-ELSD

作　　者：孙泽东 张劲松[1] 周靖 王晨光 王金艳[1] 唐庆九[1] SUN Zedong;ZHANG Jingsong;ZHOU Jing;WANG Chenguang;WANG Jinyan;TANG Qingjiu(Key Laboratory of Resources and Utilization of Edible Fungi(South),Ministry of Agriculture and Rural Affairs,National Engineering Research Center of Edible Fungi,Key Laboratory of Agriculture Genetics and Breeding of Shanghai,Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences,Shanghai 201403,China;College of Food Science,Shanghai Ocean University,Shanghai 201306,China;Shanghai Baixin Biotechnology Co.,Ltd.,Shanghai 201403,China)

机构地区：[1]上海市农业科学院食用菌研究所农业农村部南方食用菌资源利用重点实验室国家食用菌工程技术研究中心上海市农业遗传育种重点实验室,上海201403 [2]上海海洋大学食品学院,上海201306 [3]上海百信生物科技有限公司,上海201403

出　　处：《菌物学报》2023年第9期1993-2000,共8页Mycosystema

摘　　要：为准确测定灵芝孢子粉产品的酸价,阐明影响酸价的关键因素,本试验建立了高效液相色谱-蒸发光散射检测器(high performance liquid chromatography-evaporative light scattering detector,HPLC-ELSD)分析方法测定孢子油中游离脂肪酸的含量。通过优化色谱条件,建立了亚油酸、棕榈酸和油酸的HPLC的定量测定方法。该方法精密度、重复性和稳定性好,可用于灵芝孢子粉、孢子油及其相关产品中亚油酸、棕榈酸和油酸成分的定量测定。与国标化学法测定的结果比较,两种方法的匹配度较好,HPLC-ELSD法灵敏度高,可以测定不同脂肪酸的含量。通过比较分析发现,贮存时间对破壁灵芝孢子粉脂肪酸影响最大,同时产地和采收时间也影响酸价的大小。对8种不同品牌市售灵芝孢子油产品的脂肪酸含量测定,发现脂肪酸含量相差较大,其中含量最高的为(99.81±10.21)mg/g,最低的为(1.90±0.12)mg/g。因此新建立的HPLC-ELSD法可精确定量灵芝孢子粉中游离脂肪酸,为市场上灵芝孢子类产品的质量控制提供科学依据。In order to accurately determine the acid value of Ganoderma lingzhi spore powder products and clarify the key factors affecting acid value,a high performance liquid chromatography-evaporative light scattering detector(HPLC-ELSD)analysis method was established to determine the content of free fatty acids in spore oil.By optimizing the chromatographic conditions,a HPLC quantitative determination method for determining linoleic acid,palmitic acid and oleic acid was established.This method had good precision,repeatability and stability,and could be used for the quantitative determination of linoleic acid,palmitic acid and oleic acid components in G.lingzhi spore powder,spore oil and related products.Compared with the national standard chemical method,the matching degree of the HPLC-ELSD is better,and the method has high sensitivity and can determine the content of different fatty acids.Through comparative analysis,it was found that the storage time had the greatest effect on the fatty acid content of G.lingzhi spore powder,and the producing area and harvest time also affected the size of acid price.The fatty acid content of eight different brands of commercially available G.lingzhi spore oil products showed varied greatly,ranging from(1.90±0.12)mg/g to(99.81±10.21)mg/g.The newly established HPLC-ELSD method can accurately quantify the free fatty acids in G.lingzhi spore powder,which provides a scientific basis for the quality control of G.lingzhi spore products on the market.

关 键 词：灵芝孢子粉 高效液相色谱-蒸发光散射检测器(HPLC-ELSD) 脂肪酸 酸价 

分 类 号：R284.1[医药卫生—中药学]