2011-2020年福建省手足口病相关柯萨奇病毒A组8型和12型分子流行病学特征分析  

Molecular epidemiological characteristics of Coxsackieviruses A8 and A12 assciated with hand,foot,mouth disease in Fujian Province,China,2011-2020

作　　者：朱颖[1] 李林丰 何文祥[1] 翁育伟[1,2] 陈炜[1,2] ZHU Ying;LI Linfeng;HE Wenxiang;WENG Yuwei;CHEN Wei(Fujian Provincial Center for Disease Control and Prevention,Fujian Provincial Key Laboratory of Zoonosis Research,Fuzhou 350012 China;School of Public Health,FujianMedical University)

机构地区：[1]福建省疾病预防控制中心,福建省人兽共患病研究重点实验室,福建福州350012 [2]福建医科大学公共卫生学院

出　　处：《中国病原生物学杂志》2023年第11期1269-1273,共5页Journal of Pathogen Biology

基　　金：国家科技重大专项课题(No.2017ZX10104001);福建省卫生健康科技计划项目(No.2021GGB012);福建省疾病预防控制中心科研专项项目(No.2022QN01)。

摘　　要：目的研究福建省2011-2020年手足口病(hand foot and mouth disease,HFMD)相关柯萨奇病毒A组8型(CVA8)和12型(CVA12)型的流行病学及全基因组序列特征。方法对2011-2020年福建省疾病预防控制中心实验室收集的HFMD相关CVA8和CVA12病例进行流行病学特征分析;采用MegaⅩ和Simplot 3.5.1等软件对病毒VP1区和全基因组序列遗传进化分析和重组分析。结果2011-2020年福建省HFMD相关CVA8和CVA12感染各7例。获得4株CVA8完整VP1基因福建分离株,其中2株属于D基因型,2株属于E基因型;获得CVA12完整VP1基因福建分离株6株,其中2株属于B1基因亚型,4株属于B2基因亚型。比对CVA8和CVA12全基因组序列与其原型株核苷酸相似性分别为68.7%~77.7%和69.1%~78%。结合P2和P3区进化树和重组分析结果显示,CVA12-2011FJQZ168在P3区与EVA71可能发生重组,CVA8-2011FJQZ146在P2-P3区与CVA7、在P3区与CVA12-2011FJQZ168可能发生重组。结论2011-2020年福建省发现散发CVA8和CVA12感染相关手足口病病例,CVA8和CVA12福建分离株在系统进化树均存在不同的进化分支,在非结构区域存在疑似重组现象。需进一步加强对CVA8和CVA12的监测。Objective To study the molecular epidemiological characteristics of Coxsackie A 8(CVA8)and CVA12 associated with hand,foot and mouth disease(HFMD)in Fujian Province from 2011 to 2020.Methods The epidemiological characteristics of CVA8 and CVA12 that collected by the laboratory of Fujian Provincial Center for Disease Control and Prevention from 2011 to 2020 were analyzed,and phylogenetic of VP1 region and genome sequences were performed by Mega X and recombination analysis were performed by Simplot 3.5.1.Results From 2011 to 2020,seven cases of CVA8 and CVA12 were found in HFMD,respectively.Four CVA8 complete VP1 gene of Fujian isolates were obtained,two of which belonged to D genotype and two belonged to E genotype.Six CVA12 complete VP1 gene of Fujian isolates were obtained,two of which belonged to Bl subgenotype,and four belonged to B2 subgenotype.The nucleotide similarities between CVA8 and CVA12 and their own prototypes were found to be 68.7%-77.7%and 69.1%-78%,respectively.When compared to other prototypes of enterovirus A,the nucleotide similarities of certain fragments in the non-structural protein region were higher than those of their own prototypes.Combining the results of phylogenetic analysis of P2 and P3 regions and the recombination analysis,it showed that CVA12-2011FJQZ168 was probably recombined with EVA71 in P3 region,CVA8-2011FJQZ146 was probably recombined with CVA7 in P2 and P3 region and CVA12-2011FJQZ168 in partial P3 region.C.onclusion From 2011 to 2020,sporadic cases of HFMD related to CVA8 or CVA12 infections were found in Fujian Province.The CVA8 and CVA12 isolates in Fujian have different evolutionary branches,respectively.And the suspected recombination may occured in the non-structural protein region.The surveil lance of CVA8 and CVA12 should be further strengthened.

关 键 词：手足口病 柯萨奇病毒A8 柯萨奇病毒A12 分子流行病学 

分 类 号：R373.2[医药卫生—病原生物学]