细粒棘球绦虫糖转运体早期响应脱水蛋白的生物信息学及系统发育分析  

Bioinformatic analysis and phylogenetic tree of ERD6 of Echinococcus granulosus

作　　者：周润 周婧 许少全 颜明智 吕国栋 ZHOU Run;ZHOU Jing;XU Shaoquan;YAN Mingzhi;LV Guodong(College of Pharmacy,Xinjiang Medical University,Urumqi 830054,China;State Key Laboratory of Pathogenesis,Prevention,and Treatment of Central Asian High Incidence Diseases,Clinical Medical Research Institute,First Affiliated Hospital of XinjiangMedical University)

机构地区：[1]新疆医科大学药学院,新疆乌鲁木齐830054 [2]新疆医科大学第一附属医院,临床医学研究院,省部共建中亚高发病成因与防治国家重点实验室

出　　处：《中国病原生物学杂志》2023年第11期1296-1302,1310,共8页Journal of Pathogen Biology

基　　金：新疆维吾尔自治区自然科学基金项目(No.2022D01E66)。

摘　　要：目的克隆细粒棘球绦虫糖转运体早期响应脱水蛋白(Sugar transporter early responsive to dehydration 6 of Echinococcus granulosus,EgERD6)基因序列,并进行生物信息学分析。方法从细粒棘球绦虫中通过RT-PCR方法扩增EgERD6基因全长序列,结合生物信息学方法预测EgERD6蛋白分子的生物学特性,并进行系统发育、同源序列比对以及多蛋白相互作用关系分析。结果克隆获得EgERD6基因全长序列,共1470 bp,编码488 aa蛋白,该蛋白具有主要协同转运蛋白超家族(Major facilitator superfamily,MFS)特征结构域。生物信息学分析该蛋白为疏水性蛋白,二级结构以α螺旋为主,约占50.92%。氨基酸序列比对显示该蛋白与多房棘球绦虫的同源性为97.48%,与其他物种同源性较低。系统进化分析显示,细粒棘球绦虫ERD6与人ERD6亲缘关系较远。该蛋白可与细粒棘球绦虫内啡肽-A3、网格蛋白、肝细胞生长因子调节的酪氨酸激酶底物等多种蛋白质共同参与内吞作用和囊泡介导转运等生物学过程。结论成功克隆了EgERD6基因,生物信息学分析其编码蛋白含有MFS保守的功能结构域,可能参与虫体多种重要物质及能量代谢过程,为囊型棘球蚴病的致病机制研究和新的临床治疗方案的探索奠定了基础。Objective To obtain the full-length sequence of the ERD6 gene and to clone and bioinformatically analyze the sequence of the sugar transporter early responsive to dehydration 6 of Echinococcus granulosus(EgERD6)gene.Methods The amplification of the complete length of the EgERD6 gene was achieved through RT-PCR using E.granulosus as the source.Subsequently,the target gene was isolated and purified,followed by the construction of the pMD19-TERD6 plasmid.This plasmid was then transformed into Escherichia coli DH5αreceptor cell culture,and the resulting plasmids were extracted and subjected to enzymatic profiling and PCR for identification purposes.Bioinformatics techniques were employed to forecast the biological characteristics of EgERD6 protein molecules,encompassing phylogenetic analysis,homologous sequence comparison,and multiprotein interaction investigation.Results The complete sequence of EgERD6,consisting of 1,470 base pairs,was successfully cloned.This sequence encodes a protein of 488 amino acids,which exhibits the characteristic domain of the Major facilitator superfamily(MFS)and contains 12 transmembrane regions.The results of a systematic evolutionary analysis indicate a close relationship between ERD6 of E.granulosus and ERD6 of Homo sapiens.Furthermore,the findings from protein-protein interaction studies suggest that this protein has the potential to be involved in various biological processes,such as endocytosis and vesico-mediated transport,including the regulation of endorphin-A3,lattice protein,and tyrosine kinase substrate by hepatocyte growth factor(HGF).Conclusion The EgERD6 gene was successfully cloned and bioinformatically analysed to have a conserved functional structural domain of MFS,which may be involved in a variety of important material and energy metabolic processes in the worm,laying the foundation for studying the pathogenesis of cystic echinococcosis and exploring new clinical treatment options.

关 键 词：细粒棘球绦虫 糖转运体早期响应脱水蛋白 生物信息学分析 

分 类 号：R383.3[医药卫生—医学寄生虫学]