The photosensory function of Zmphot1 differs from that of Atphot1 due to the C-terminus of Zmphot1 during phototropic response  

作　　者：Jindong Zhu Fangyuan Zhou Yuxi Wang Yuping Liang Qingping Zhao Yuanji Han Xiang Zhao 

机构地区：[1]State Key Laboratory of Crop Stress Adaptation and Improvement,School of Life Sciences,Henan University,Kaifeng 475004,Henan,China

出　　处：《The Crop Journal》2023年第5期1331-1340,共10页作物学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(31871419, 32200252, and 32100225);the Program for Innovative Research Team (in Science and Technology) at University of Henan Province (21IRTSTHN019);the Henan Overseas Expertise Introduction Center for Discipline Innovation (CXJD2020004);the Natural Science Foundation of Henan Province (212300410214)。

摘　　要：The role of phot1 in triggering hypocotyl phototropism and optimizing growth orientation has been wellcharacterized in Arabidopsis, whereas the role of Zmphot1 in maize remains largely unclear. Here, we show that Zmphot1 is involved in blue light-induced phototropism. Compared with Atphot1, Zmphot1exhibited a weaker phototropic response to very low-fluence rates of blue light(< 0.01 μmol m-2s-1),but stronger phototropic response to high-fluence rates of blue light(> 10 μmol m-2s-1) than Atphot1. Notably, blue light exposure induced Zmphot1-green fluorescent protein(GFP), but not Atphot1-GFP, to form the aggregates in the cytoplasm of Nicotiana benthamiana cells. Furthermore, by generating the chimeric phot1 proteins, we found that the serine-threonine kinase(STK) domain at the C-terminus is responsible for a more volatile membrane association of Zmphot1. Consistently, the chimeric phot1 protein fusing the STK domain of Zmphot1 with other domains of Atphot1 responded similarly as Zmphot1 to both low and high fluence rates of blue light. Interestingly, although both Zmphot1 and Atphot1 interact with AtNPH3, Zmphot1 induced weaker dephosphorylation of NONPHOTOTROPIC HYPOCOTYL 3(NPH3) than Atphot1. Together, our findings indicate that Zmphot1 and Atphot1 exhibit different photosensory function during phototropic response and that the STK domain may play a key role in determining their properties.

关 键 词：Blue light Zmphot1 Atphot1 Hypocotyl phototropism 

分 类 号：S311[农业科学—作物栽培与耕作技术]