Fine mapping and validation of a stable QTL for thousand-kernel weight in wheat(Triticum aestivum L.)  

作　　者：Deyuan Meng Aamana Batool Yazhou Xuan Ruiqing Pan Na Zhang Wei Zhang Liya Zhi Xiaoli Ren Wenqing Li Jijie Li Yanxiao Niu Shuzhi Zheng Jun Ji Xiaoli Shi Lei Wang Hongqing Ling Chunhua Zhao Fa Cui Xigang Liu Junming Li Liqiang Song 

机构地区：[1]Center for Agricultural Resources Research,Institute of Genetics and Developmental Biology,The Innovative Academy of Seed Design,Chinese Academy of Sciences,Shijiazhuang 050022,Hebei,China [2]Ministry of Education Key Laboratory of Molecular and Cellular Biology,Hebei Research Center of the Basic Discipline of Cell Biology,Hebei Key Laboratory of Molecular and Cellular Biology,College of Life Sciences,Hebei Normal University,Shijiazhuang 050024,Hebei,China [3]State Key Laboratory of North China Crop Improvement and Regulation,College of Agronomy,Hebei Agricultural University,Baoding 071000,Hebei,China [4]State Key Laboratory of Plant Cell and Chromosome Engineering,Chinese Academy of Sciences,Beijing 100101,China [5]Key Laboratory of Molecular Module-Based Breeding of High Yield and Abiotic Resistant Plants in Universities of Shandong,School of Agriculture,Ludong University,Yantai 264025,Shandong,China [6]Hebei Collaboration Innovation Center for Cell Signaling,Shijiazhuang 050024,Hebei,China [7]College of Life Science,University of Chinese Academy of Sciences,Beijing 100049,China

出　　处：《The Crop Journal》2023年第5期1491-1500,共10页作物学报（英文版）

基　　金：jointly supported by the National Natural Science Foundation of China(32272056,U22A6009,31671673,and 31871612);Hebei Natural Science Foundation(C2021205013,C2022204202);Talents Program of Hebei Agricultural University in China(YJ2021016);China Agriculture Research System of MOF and MARA(CARS-03)。

摘　　要：Thousand-kernel weight(TKW)is a measure of grain weight,a target of wheat breeding.The object of this study was to fine-map a stable quantitative trait loci(QTL)for TKW and identify its candidate gene in a recombinant inbred line(RIL)population derived from the cross of Kenong 9204(KN9204)and Jing411(J411).On a high-density genetic linkage map,24,26 and 25 QTL were associated with TKW,kernel length(KL),and kernel width(KW),respectively.A major and stable QTL,QTkw-2D,was mapped to an8.3 cM interval on chromosome arm 2DL.By saturation of polymorphic markers in its target region,QTkw-2D was confined to a 9.13 Mb physical interval using a secondary mapping population derived from a residually heterozygous line(F6:7).This interval was further narrowed to 2.52 Mb using QTkw-2D near-isogenic lines(NILs).NILs~(KN9204)had higher fresh and dry weights than NILsJ411at various grain-filling stages.The TKW and KW of NILs~(KN9204)were much higher than those of NILsJ411in field trials.By comparison of both DNA sequence and expression between KN9204 and J411,TraesCS2D02G460300.1(TraesKN2D01HG49350)was assigned as a candidate gene for QTkw-2D.This was confirmed by RNA sequencing(RNA-seq)of QTkw-2D NILs.These results provide the basis of map-based cloning of QTkw-2D,and DNA markers linked to the candidate gene may be used in marker-assisted selection.

关 键 词：WHEAT Thousand-kernel weight Fine mapping Candidate gene 

分 类 号：S512.1[农业科学—作物学]