Introgression of sharp eyespot resistance from Dasypyrum villosum chromosome 2VL into bread wheat  

作　　者：Caiyun Liu Wei Guo Yang Wang Bisheng Fu Jaroslav Doležel Ying Liu Wenling Zhai Mahmoud Said István Molnár Kateřina Holušová Ruiqi Zhang Jizhong Wu 

机构地区：[1]Institute of Germplasm Resources and Biotechnology/Jiangsu Provincial Key Laboratory of Agrobiology,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,Jiangsu,China [2]College of Agronomy/JCIC-MCP/National Key Lab of Crop Genetics and Germplasm Enhancement,Nanjing Agricultural University,Nanjing 210095,Jiangsu,China [3]Institute of Experimental Botany,Centre of the HanáRegion for Biotechnological and Agricultural Research,Šlechtitelů31,CZ-783671 Olomouc,the Czech Republic [4]Field Crops Research Institute,Agricultural Research Centre,9 Gamma Street,Giz1,12619 Cairo,Egypt [5]Agricultural Institute,Centre for Agricultural Research,ELKH,Martonvásár 2462,Hungary [6]Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops,Yangzhou University,Yangzhou 225009,Jiangsu,China [7]Zhongshan Biological Breeding Laboratory,Nanjing 210014,Jiangsu,China

出　　处：《The Crop Journal》2023年第5期1512-1520,共9页作物学报（英文版）

基　　金：supported by the National Key Research and Develpment Program of China (2021YFD1200600);the National Natural Science Foundation of China (31871619, 32101703, and 32101800);the Natural Science Foundation of Jiangsu Province (BK20210152);the Jiangsu Seed Industry Revitalization Project (JBGS (2021) 013);the Key Research and Development Program of Jiangsu Province(BE2022346);Jiangsu Agricultural Science and Technology Innovation Fund of China (CX (20) 3029);supported by the European Regional Development Fund (CZ.02.1.01/0.0/0.0/16_019/0000827)。

摘　　要：Wheat sharp eyespot, a stem disease caused by the soilborne fungus Rhizoctonia cerealis van der Hoeven,has become a threat to wheat production worldwide. Exploiting resistance resources from wild relatives of wheat is a promising strategy for controlling this disease. In this study, a new wheat–Dasypyrum villosum T2DS·2V#4L translocation line in the background of Chinese Spring(CS) showed stable resistance to R. cerealis. Introgression of the T2DS·2V#4L chromosome into wheat cultivar Aikang 58 by backcrossing produced a marked increase in sharp eyespot resistance in NIL-T2DS·2V#4L in comparison with NILT2DS·2DL, and no detrimental effects of 2V#4L on agronomic traits were observed in the BC2F2, BC2F2:3,and BC2F2:4generations. Flow-sorted sequencing of 2V#4L yielded 384.3 Mb of assembled sequence, and8836 genes were predicted of which 6154 had orthologs in at least one of the 2AL, 2BL, and 2DL arms of CS, whereas 1549 genes were unique to 2V#4L. About 100,000 SNPs were detected in genes of 2V#4L and2DL in 10 sequenced bread wheat cultivars. A Kompetitive Allele Specific Polymerase chain reaction and30 conserved ortholog sequence markers were developed to trace the 2V#4L chromatin in wheat backgrounds. T2DS·2V#4L compensating translocation lines represent novel germplasm with sharp eyespot resistance and the markers will allow rapid detection in breeding programs.

关 键 词：Triticum aestivum Rhizoctonia cerealis Dasypyrum villosum Flow sorting KASP 

分 类 号：S435.12[农业科学—农业昆虫与害虫防治]