Characterization of meiotic chromosome behavior in the autopolyploid Saccharum spontaneum reveals preferential chromosome pairing without distinct DNA sequence variation  

作　　者：Xin Zhang Zhuang Meng Jinlei Han Haris Khurshid Ayman Esh Robert Hasterok Kai Wang 

机构地区：[1]School of Life Sciences,Nantong University,Nantong 226019,Jiangsu,China [2]Plant Cytogenetics and Molecular Biology Group,Institute of Biology,Biotechnology and Environmental Protection,Faculty of Natural Sciences,University of Silesia in Katowice,Katowice 40-032,Poland [3]College of Agriculture,Fujian Agriculture and Forestry University,Fuzhou 350002,Fujian,China [4]Sugar Crops Research Institute,Agriculture Research Center,Giza 12111,Egypt [5]Oilseeds Research Program,National Agricultural Research Centre,Islamabad 44000,Pakistan

出　　处：《The Crop Journal》2023年第5期1550-1558,共9页作物学报（英文版）

基　　金：funded by the Startup Foundation from Nantong University (03083074);partially supported by the National Natural Science Foundation of China (31771862);Special Funds for Technology Innovation of Fujian Agriculture and Forestry University(KFA20001A);the Research Program of Guangxi Key Laboratory for Sugarcane Biology (GXKLSCB-20190203)。

摘　　要：Autopolyploidy and allopolyploidy may represent an evolutionary advantage and are more common in plants than assumed. However, less attention has been paid to autopolyploidy than to allopolyploidy,and its evolutionary consequences are largely unclear, especially for plants with high ploidy levels. In this study, we developed oligonucleotide(oligo)-based chromosome painting probes to identify individual chromosomes in S. spontaneum. Using fluorescence in situ hybridization(FISH), we investigated chromosome behavior during pachytene, metaphase, anaphase, and telophase of meiosis I(MI) in autotetraploid,autooctoploid, and autodecaploid S. spontaneum clones. All autopolyploid clones showed stable diploidized chromosome behavior;so that homologous chromosomes formed almost exclusively bivalents during MI. Two copies of homologous chromosome 8 with similar sizes in the autotetraploid clone showed preferential pairing with each other with respect to the other copies. However, sequence variation analysis showed no apparent differences among homologs of chromosome 8 and all other chromosomes. We suggest that either the stable diploidized pairing or the preferential pairing between homologous copies of chromosome 8 in the studied autopolyploid sugarcane are accounted for by unknown mechanisms other than DNA sequence similarity. Our results reveal evolutionary consequences of stable meiotic behavior in autopolyploid plants.

关 键 词：AUTOPOLYPLOIDY Saccharum spontaneum Meiosis I Chromosome behavior Chromosome pairing 

分 类 号：S566.1[农业科学—作物学]